Varshavskiĭ V A, Ponomarev A B, Bazarova A V, Karelin A A, Pankov Iu A, Kondrat'ev Ia Iu, Keda Iu M, Gorskova V A
Arkh Patol. 1990;52(7):43-8.
Kidneys of 16 Wistar rats were examined by light and electron microscopy, immunofluorescence and biochemically for the transamidinase activity at various periods of experimental diabetes induced by the fractionated intraperitoneal administration of low (40 mg/kg) doses of streptozotocin. 18 rats of the same age and sex served as control. This model of diabetes is characterized by a gradual decrease of the serum immunoreactive insulin, increase of hyperglycemia, the presence of "insulitis" 19 days after the beginning of the experiment and the development of nephropathy in the genesis of which immune mechanisms might participate. Transamidinase activity correlated with the alterations of renal tubuli. The conclusion is made on the possibility of using this model of experimental diabetes for studying the pathogenetic mechanisms of renal lesions in diabetes; transamidinase activity allows one to evaluate the nephron function in diabetic nephropathy.
通过光学显微镜和电子显微镜、免疫荧光以及生化方法，对16只经腹腔分次注射低剂量（40mg/kg）链脲佐菌素诱导实验性糖尿病不同时期的Wistar大鼠的肾脏进行了检查，以检测转脒基酶活性。选取18只相同年龄和性别的大鼠作为对照。该糖尿病模型的特点是血清免疫反应性胰岛素逐渐降低、高血糖症加重、实验开始19天后出现“胰岛炎”以及可能涉及免疫机制的肾病发生。转脒基酶活性与肾小管的改变相关。得出结论，该实验性糖尿病模型可用于研究糖尿病肾脏病变的发病机制；转脒基酶活性可用于评估糖尿病肾病中的肾单位功能。
