Tesch Greg H, Allen Terri J
Department of Nephrology, Monash University, Monash Medical Centre, Clayton, Victoria, Australia.
Nephrology (Carlton). 2007 Jun;12(3):261-6. doi: 10.1111/j.1440-1797.2007.00796.x.
Streptozotocin-induced pancreatic injury is commonly used for creating rodent models of type 1 diabetes which develop renal injury with similarities to human diabetic nephropathy. This model can be established in genetically modified rodents for investigating the role of molecular mechanisms and genetic susceptibility in the development of diabetic nephropathy. In this report, the authors describe and compare the current protocols being used to establish models of diabetic nephropathy in rat and mouse strains using streptozotocin. The authors also list some of the histological criteria and biochemical measurements which are being used to validate these models. In addition, our review explains some of the key aspects involved in these models, including the impact of streptozotocin-dosage, uninephrectomy, hypertension and genetically modified strains, which can each affect the development of disease and the interpretation of findings.
链脲佐菌素诱导的胰腺损伤常用于建立1型糖尿病啮齿动物模型，该模型会发展出与人类糖尿病肾病相似的肾损伤。这种模型可以在基因改造的啮齿动物中建立，用于研究分子机制和遗传易感性在糖尿病肾病发展中的作用。在本报告中，作者描述并比较了目前使用链脲佐菌素在大鼠和小鼠品系中建立糖尿病肾病模型的方案。作者还列出了一些用于验证这些模型的组织学标准和生化测量方法。此外，我们的综述解释了这些模型涉及的一些关键方面，包括链脲佐菌素剂量、单侧肾切除、高血压和基因改造品系的影响，这些因素都会影响疾病的发展和研究结果的解释。
