South China Agricultural University, Guangzhou 510642, People's Republic of China.
Ecotoxicol Environ Saf. 2011 Jul;74(5):1348-54. doi: 10.1016/j.ecoenv.2011.03.011. Epub 2011 Apr 15.
The ultrastructure of Nilaparvata lugens brain cells was damaged by treatment at different fipronil concentrations. The cell showed swollen mitochondria and vacuolization, but no mitochondrial cristae. Rough endoplasmic reticulum (RER) fragmentation and degranulation were seen. The dilatation of endoplasmic reticulum cisterns was very prominent, and the predominant lamellar RERs were arranged chaotically. The Golgi apparatus demonstrated obvious changes in configuration, as dilated with closed cisternae and atypical vesicles. The mitochondria mainly showed large vacuolization in muscles. Nuclear degeneration and condensation and increased numbers of large hydropic vacuoles and lysosomes were observed. It was concluded that the effect on cellular components was fipronil-specific. Changes in cellular ultrastructure seem to be an appropriate ecotoxicological indicator of the insecticide's efficacy.
褐飞虱脑细胞的超微结构在不同吡虫啉浓度处理下受到损伤。细胞显示肿胀的线粒体和空泡化，但没有线粒体嵴。粗面内质网（RER）碎片化和脱粒可见。内质网池的扩张非常明显，主要的层状 RER 排列杂乱。高尔基器在形态上表现出明显的变化，出现扩张的封闭小池和非典型小泡。线粒体在肌肉中主要表现为大的空泡化。核变性和浓缩，以及大量大的含水空泡和溶酶体增加。结论是，对细胞成分的影响是吡虫啉特异性的。细胞超微结构的变化似乎是杀虫剂功效的适当生态毒理学指标。
