Department of Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Vet Microbiol. 2011 Nov 21;153(1-2):156-62. doi: 10.1016/j.vetmic.2011.03.023. Epub 2011 Mar 26.
The aim of the current study was to look for evidence of possible cross-species transmission of Brachyspira species between rodents and farm animals. To do this, previously collected and characterised Brachyspira isolates from rodents, pigs and chickens on the same farms were analysed by random amplified polymorphic DNA (RAPD). Isolates with similar RAPD banding patterns were further typed by pulsed-field gel electrophoresis (PFGE). Identical isolates of Brachyspira pilosicoli, Brachyspira intermedia, Brachyspira murdochii and Brachyspira innocens from pigs and rodents and of B. murdochii from laying hens and rodents were found, indicating cross-species transmission at farm level. PFGE data from rodent isolates of Brachyspira hyodysenteriae were compared with PFGE data from previously typed field isolates of B. hyodysenteriae from pigs with swine dysentery and isolates from mallards (Anas platyrhynchos). Three of four isolates of B. hyodysenteriae from rodents were similar to porcine field isolates by PFGE. PCR analyses of the plasmid-encoded and potential virulence determinants rfb genes B, A, D and C showed that they were present in isolates of B. hyodysenteriae of porcine, mallard and rodent origin.
本研究旨在寻找啮齿动物和农场动物之间可能发生的短螺旋体属种间交叉传播的证据。为此，对来自同一农场的啮齿动物、猪和鸡的先前收集和特征化的短螺旋体属分离株进行随机扩增多态性 DNA（RAPD）分析。具有相似 RAPD 带型的分离株进一步通过脉冲场凝胶电泳（PFGE）进行分型。从猪和啮齿动物中发现了相同的猪痢疾密螺旋体、中间密螺旋体、穆尔氏密螺旋体和无害密螺旋体的分离株，以及来自产蛋鸡和啮齿动物的穆尔氏密螺旋体的分离株，表明在农场水平上发生了种间传播。将来自猪痢疾密螺旋体的啮齿动物分离株的 PFGE 数据与先前从患有猪痢疾的猪和来自野鸭（Anas platyrhynchos）的猪痢疾密螺旋体场分离株中分型的 PFGE 数据进行比较。来自啮齿动物的 4 个 B. hyodysenteriae 分离株中有 3 个通过 PFGE 与猪场分离株相似。对质粒编码和潜在毒力决定簇 rfb 基因 B、A、D 和 C 的 PCR 分析表明，它们存在于源自猪、野鸭和啮齿动物的 B. hyodysenteriae 分离株中。
