新型微流控盘在单细胞活力分析中的应用及在 Jurkat 细胞中的应用。

Use of a novel microfluidic disk in the analysis of single-cell viability and the application to Jurkat cells.

机构信息

Department of Bioinformatics, Graduate School of Engineering, Soka University, Hachioji, Tokyo 192-8577, Japan.

出版信息

J Biosci Bioeng. 2011 Jul;112(1):98-101. doi: 10.1016/j.jbiosc.2011.03.016. Epub 2011 Apr 16.

DOI:10.1016/j.jbiosc.2011.03.016

Abstract

Jurkat cells were trapped in the microchambers of a novel disk-shaped cell separation device and stained with Cellstain. Approximately 90% of the cells were living. Single cells were isolated with a branching microchannel after rotation at 4500rpm for 30s, demonstrating that a living single cell could be trapped in the microchambers.

摘要

Jurkat 细胞被捕获在新型盘状细胞分离装置的微室中，并使用 Cellstain 进行染色。大约 90%的细胞是存活的。在以 4500rpm 旋转 30s 后，通过分支微通道分离单细胞，表明活的单个细胞可以被捕获在微室中。