编码家蚕和玉米螟 p53 的 cDNA 的鉴定。

Characterization of cDNAs encoding p53 of Bombyx mori and Spodoptera frugiperda.

机构信息

Division of Biology, Kansas State University, Manhattan, KS 66503, USA.

出版信息

Insect Biochem Mol Biol. 2011 Aug;41(8):613-9. doi: 10.1016/j.ibmb.2011.03.014. Epub 2011 Apr 8.

DOI:10.1016/j.ibmb.2011.03.014

Abstract

Complementary DNAs encoding homologs of the tumor suppressor gene, p53, were characterized from two lepidopteran insects, Bombyx mori (Bm) and Spodoptera frugiperda (Sf). They encoded predicted proteins of 368 (41.2 kDa) (Bm) and 374 (42.5 kDa) (Sf) amino acids. The sequences shared 44% amino acid and 60% nucleotide sequence identity with each other, but exhibited less than 20% amino acid and 46% nucleotide sequence identity to Drosophila melanogaster p53. Despite the sequence diversity, conserved amino acids involved in DNA and zinc binding were present in the lepidopteran sequences. Expression of Sfp53-induced apoptosis in S. frugiperda cells, and antiserum made against recombinant Sfp53 recognized a protein whose abundance increased after treatment with DNA damaging agents.

摘要

互补 DNA 编码的肿瘤抑制基因 p53 同源物，从两种鳞翅目昆虫，家蚕（Bm）和草地贪夜蛾（Sf）中得到了鉴定。它们编码的预测蛋白分别为 368（41.2 kDa）（Bm）和 374（42.5 kDa）（Sf）个氨基酸。这些序列彼此之间具有 44%的氨基酸和 60%的核苷酸序列同一性，但与果蝇 p53 的氨基酸序列同一性小于 20%，核苷酸序列同一性小于 46%。尽管存在序列多样性，但在鳞翅目序列中存在涉及 DNA 和锌结合的保守氨基酸。在 S. frugiperda 细胞中表达 Sf p53 诱导细胞凋亡，并且针对重组 Sf p53 产生的抗血清识别一种在 DNA 损伤剂处理后丰度增加的蛋白。