Beijing National Laboratory for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China.
Colloids Surf B Biointerfaces. 2011 Aug 1;86(1):81-6. doi: 10.1016/j.colsurfb.2011.03.019. Epub 2011 Mar 23.
Enhanced sensitivity for the hepatotoxin microcystin-LR (MC-LR) was achieved in a quartz crystal microbalance (QCM) system via double amplification. For primary amplification, an innovative interface on the QCM was obtained as a matrix by the vesicle layer formed by our synthetic dendritic surfactant, bis (amidoethyl-carbamoylethyl) octadecylamine (C18N3). The vesicle matrix was then functionalised by an optimised concentration of monoclonal antibodies against MC-LR (anti-MC-LR) to detect the analyte. The results showed that a detection limit of 100 ng/mL was achieved by primary amplification. To achieve higher sensitivity, secondary amplification was implemented with anti-MC-LR gold nanoparticle (AuNPs) conjugates as probes, which lowered the detection limit for MC-LR to 1 ng/mL (the maximum concentration recommended by the World Health Organization [WHO] in drinking water for humans). The QCM immunosensor reported here has advantages such as high sensitivity, portability, simplicity, and cost-effectiveness for MC-LR detection. It would be uniquely superior compared with current MC-LR detection techniques for on-the-spot water detection. Furthermore, the methodology described here is also potentially significant in many fields for the routine monitoring of environmental and food safety.
通过双重放大，石英晶体微天平（QCM）系统实现了对肝毒素微囊藻毒素-LR（MC-LR）的高灵敏度检测。作为初步放大，我们合成的树枝状表面活性剂双（酰胺乙基-氨甲酰乙基）十八烷基胺（C18N3）形成的囊泡层作为基质，在 QCM 上获得了一种创新的界面。然后，通过优化的针对 MC-LR 的单克隆抗体（抗 MC-LR）浓度对囊泡基质进行功能化，以检测分析物。结果表明，通过初步放大实现了 100ng/mL 的检测限。为了实现更高的灵敏度，采用抗 MC-LR 金纳米粒子（AuNPs）缀合物作为探针进行二次放大，将 MC-LR 的检测限降低至 1ng/mL（世界卫生组织[WHO]推荐的人类饮用水中最大浓度）。与目前用于现场水质检测的 MC-LR 检测技术相比，本研究报告的 QCM 免疫传感器具有灵敏度高、便携性、简单性和成本效益等优点。它在许多领域对于环境和食品安全的常规监测也具有潜在的重要意义。
