National Food Research Institute, National Agriculture and Food Research Organization (NARO), 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
Bioresour Technol. 2011 Jun;102(11):6502-7. doi: 10.1016/j.biortech.2011.03.065. Epub 2011 Mar 26.
A simple process (the direct-saccharification-of-culms (DiSC) process) to produce ethanol from rice straw culms, accumulating significant amounts of soft carbohydrates (SCs: glucose, fructose, sucrose, starch and β-1,3-1,4-glucan) was developed. This study focused on fully mature culms of cv. Leafstar, containing 69.2% (w/w of dried culms) hexoses from SCs and cellulose. Commercially-available wind-separation equipment successfully prepared a culm-rich fraction with a SC recovery of 83.1% (w/w) from rice straw flakes (54.1% of total weight of rice straw). The fraction was suspended in water (20%, w/w) for starch liquefaction, and the suspension was subjected to a simultaneous saccharification and fermentation with yeast, yielding 5.6% (w/v) ethanol (86% of the theoretical yield from whole hexoses in the fraction) after 24h fermentation. Thus, the DiSC process produced highly-concentrated ethanol from rice straw in a one vat process without any harsh thermo-chemical pretreatments.
开发了一种从水稻秸秆中生产乙醇的简单工艺（直接糖化秸秆工艺，DiSC 工艺），该工艺可积累大量的软碳水化合物（SCs：葡萄糖、果糖、蔗糖、淀粉和β-1,3-1,4-葡聚糖）。本研究以完全成熟的 Leafstar 品种的秸秆为研究对象，其 SC 和纤维素中含有 69.2%（干秸秆重量的百分比）的己糖。商用风选设备成功地从水稻秸秆片（占水稻秸秆总重量的 54.1%）中制备了富含秸秆的部分，SC 回收率为 83.1%（w/w）。将该部分悬浮在水中（20%，w/w）进行淀粉液化，然后在酵母存在下进行同步糖化和发酵，24h 发酵后可得到 5.6%（w/v）乙醇（该部分中总己糖理论产量的 86%）。因此，DiSC 工艺在一个罐式工艺中无需任何苛刻的热化学预处理，即可从水稻秸秆中生产高浓度乙醇。
