Regenerative Medicine Program, Sprott Centre for Stem Cell Research, Ottawa Hospital Research Institute, 501 Smyth Road, Ottawa, ON K1Y 8L6, Canada.
Stem Cell Rev Rep. 2011 Nov;7(4):1041-5. doi: 10.1007/s12015-011-9264-4.
The hair follicles develop from a stem cell population in the surface ectoderm that feeds a complexe terminal differentiation pathway. We have developed a two-step high density culture scheme in which pluripotent mouse ES cells are induced first to ectoderm phenotype and then give rise to morphologically three dimensional nodule-like structures that express hair keratin in the center of them suggesting that they are progressing along the terminal differentiation program of the hair follicle in vitro. Using this model system we have now analyzed the protein expression profile using a high throughput western blotting method (BP Power Blot). This protocol provides an invaluable system in which to study both the mechanisms that direct stem cells along the hair follicle pathway as well as those that influence their subsequent epidermal differentiation in vitro.
毛囊由表皮外胚层中的干细胞群发育而来，这些细胞群为复杂的终末分化途径提供营养。我们开发了一种两步高密度培养方案，其中多能小鼠 ES 细胞首先被诱导为外胚层表型，然后形成形态上呈三维结节状结构，在它们的中心表达毛发角蛋白，表明它们在体外沿着毛囊的终末分化程序进行。使用这个模型系统，我们现在使用高通量 Western 印迹法（BP Power Blot）分析了蛋白质表达谱。该方案提供了一个非常有价值的系统，可用于研究指导干细胞沿着毛囊途径的机制，以及影响它们在体外随后表皮分化的机制。
