Department of Hepatopancreaticobiliary Surgery, St James's University Hospital, Leeds, UK.
Proteomics. 2011 May;11(10):2134-8. doi: 10.1002/pmic.201000653. Epub 2011 Apr 18.
The need to find biomarkers for hepatobiliary diseases including cholangiocarcinoma (CCA) has led to an interest in using bile as a proximal fluid in biomarker discovery experiments, although there are inherent challenges both in its acquisition and analysis. The study described here greatly extends previous studies that have started to characterise the bile proteome. Bile from four patients with hilar CCA was depleted of albumin and immunoglobulin G and analysed by GeLC-MS/MS. The number of proteins identified per bile sample was between 378 and 741. Overall, the products of 813 unique genes were identified, considerably extending current knowledge of the malignant bile proteome. Of these, 268 were present in at least 3 out of 4 patients. This data set represents the largest catalogue of bile proteins to date and together with other studies in the literature constitutes an important prelude to the potential promise of expression proteomics and subsequent validation studies in CCA biomarker discovery.
寻找肝胆疾病（包括胆管癌）生物标志物的需求促使人们对使用胆汁作为生物标志物发现实验的近端液体产生了兴趣，尽管在获取和分析胆汁时存在固有的挑战。本研究大大扩展了之前开始描述胆汁蛋白质组的研究。从 4 名肝门部胆管癌患者的胆汁中去除白蛋白和免疫球蛋白 G，并通过 GeLC-MS/MS 进行分析。每个胆汁样本中鉴定的蛋白质数量在 378 到 741 之间。总体而言，鉴定出了 813 个独特基因的产物，大大扩展了目前对恶性胆汁蛋白质组的认识。其中，268 个在至少 4 个患者中的 3 个以上存在。该数据集代表了迄今为止最大的胆汁蛋白质目录，与文献中的其他研究一起，为表达蛋白质组学在胆管癌生物标志物发现中的潜在应用和后续验证研究构成了重要的前奏。
