Laboratory of Biomaterials, School of Chinese Medicine, China Medical University, Taichung, Taiwan.
Macromol Biosci. 2011 Jul 7;11(7):914-26. doi: 10.1002/mabi.201000498. Epub 2011 Apr 15.
In this study, GCC protein was used for the first time to construct a biodegradable conduit for peripheral nerve repair. The GCC was highly stable with a sufficiently high level of mechanical properties and it was non-toxic and non-apoptotic which could maintain the survival and outgrowth of Schwann cells. Noninvasive bioluminescence imaging accompanied with histochemical assessment showed the GCC was highly biocompatible after subcutaneous implantation in transgenic mice. Electrophysiology, labeling of calcitonin gene-related peptide in the lumbar spinal cord and histology analysis also showed a rapid morphological and functional recovery for disrupted rat sciatic nerves repaired with the GCC conduits. Therefore, we conclude that the GCC can offer great nerve regeneration characteristics and can be a promising material for the successful repair of peripheral nerve defects.
在这项研究中，GCC 蛋白首次被用于构建用于周围神经修复的可生物降解导管。GCC 非常稳定，具有足够高的机械性能水平，并且无毒、无细胞凋亡，能够维持施万细胞的存活和突起生长。非侵入性生物发光成像伴随组织化学评估显示，GCC 在转基因小鼠的皮下植入后具有高度的生物相容性。电生理学、腰椎脊髓降钙素基因相关肽的标记和组织学分析也表明，用 GCC 导管修复受损的大鼠坐骨神经可实现快速的形态和功能恢复。因此，我们得出结论，GCC 可以提供出色的神经再生特性，并且是成功修复周围神经缺损的有前途的材料。
