Wang Chun-yu, Cao Li, Tang Bei-sha, Zhang Hai-nan, Guo Ji-feng, Liao Shu-sheng, Tang Jian-guang, Yan Xin-riang, Tan Li-ming
Department of Neurology, the Second Xiangya Hospital, Central South University, Changsha 410011, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2011 Mar;42(2):157-60.
To study the metabolic pathways of 2-oxoglutarate carrier protein (OGCP)and the influence of parkin protein on the metabolism of OGCP.
The OGCP metabolic pathways were identified through inhibiting proteasome activities with specific proteasome inhibitors and protease inhibitors. The isotope pulse-chase experiments were performed to measure the turnover rate of OGCP and to study the influence of parkin protein on the metabolism of OGCP.
Proteasome inhibitors and protease inhibitors inhibited OGCP degradation. The OGCP metabolism had a half-life of about 8-10 h. Overexpression of parkin protein accelerated the OGCP degradation.
OGCP degrades through proteasome and lysosome degradation pathways. The degradation of parkin protein can promote the degradation of OGCP.
研究2-氧代戊二酸载体蛋白（OGCP）的代谢途径以及帕金蛋白对OGCP代谢的影响。
通过用特异性蛋白酶体抑制剂和蛋白酶抑制剂抑制蛋白酶体活性来鉴定OGCP代谢途径。进行同位素脉冲追踪实验以测量OGCP的周转率，并研究帕金蛋白对OGCP代谢的影响。
蛋白酶体抑制剂和蛋白酶抑制剂抑制OGCP降解。OGCP代谢的半衰期约为8-10小时。帕金蛋白的过表达加速了OGCP降解。
OGCP通过蛋白酶体和溶酶体降解途径降解。帕金蛋白的降解可促进OGCP的降解。
