[维吉宁蛋白在细胞系及人类肝细胞癌中的表达]

[Expression of vigilin in cell lines and human hepatocellular carcinoma].

作者信息

Duan Shu-wang, Wei Ling, Yang Wen-li, Chen Ke-fei, Huang Wen-qing, Qin Yang

机构信息

Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2011 Mar;42(2):170-3.

PMID:21500547

Abstract

OBJECTIVE

To explore the expression status of Vigilin (high density lipoprotein binding protein) in various cell lines and human hepatocellular carcinoma (HCC) tissues.

METHODS

The expression of Vigilin was measured semiquantitatively with western blot in hepatic cancer, cervical cancer and normal cell lines. The samples of 59 hepatocellular carcinoma tissues, 59 adjacent liver tissues and 33 distant non-tumor liver tissues were collected, Vigilin expression in the above samples was detected by immunohistochemistry.

RESULTS

Vigilin expressed in all cell lines, but no expression was found in peripheral blood lymphocytes. The expression levels of Vigilin in tumor cell lines were higher than those in normal cell lines (P < 0.05). Most of the hepatic cells expressed Vigilin, but the expression levels were different (tumor tissues: 0.2226 +/- 0.054, adjacent tissues: 0.2060 +/- 0.056, distant tissues: 0.1820 +/- 0.038, P < 0.001). Highly expression of Vigilin was observed in 54% of tumor tissues, 35% adjacement tissues, and 6% of distant non-tumor tissues, respectively.

CONCLUSION

Vigilin may have relationship with HCC progression and proliferation.

摘要

目的

探讨维吉林（高密度脂蛋白结合蛋白）在多种细胞系及人肝细胞癌（HCC）组织中的表达情况。

方法

采用蛋白质免疫印迹法对肝癌、宫颈癌及正常细胞系中的维吉林表达进行半定量检测。收集59例肝细胞癌组织、59例癌旁肝组织及33例远处非肿瘤肝组织样本，采用免疫组织化学法检测上述样本中维吉林的表达。

结果

维吉林在所有细胞系中均有表达，但在外周血淋巴细胞中未检测到表达。肿瘤细胞系中维吉林的表达水平高于正常细胞系（P < 0.05）。大部分肝细胞表达维吉林，但表达水平有所不同（肿瘤组织：0.2226 ± 0.054，癌旁组织：0.2060 ± 0.056，远处组织：0.1820 ± 0.038，P < 0.001）。维吉林高表达分别见于54%的肿瘤组织、35%的癌旁组织及6%的远处非肿瘤组织。