Han Li-ping, Liu Juan-fang, Liu Xiang-rong, Dong Zi-ming, Suo Zhen-he
Department of Obstetrics and Gynecology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2011 Mar;42(2):179-84.
To investigate the expression and prognostic significance of EphA2 and EphrinA-1 in ovarian serous carcinomas.
Ninety five tumors from the patients with ovarian serous carcinomas and 2 ovarian cancer cell lines were recruited. The expressions of EphA2 and EphrinA-1 were examined by means of immunohistochemistry. The relationships among protein expression and clinicopathological features, survival of patients were analyzed. The mRNA and protein expressions of EphA2 and EphrinA-1 in ovarian cancer cell lines were measured with semiquantitative polymerase chain reaction and western blotting.
The protein expressions of EphA2 and EphrinA-1 in tumor were 92.6% (88/95)and 97.9% (93/95) respectively, while were 40%(8/20) and 30% (6/20) in adjacent ovarian tissue (P = 0.000). EphA2 immunohistochemical staining could be observed in both tumour and vascular endothelial cells, and EphrinA-1 mainly localized in the tumor cells. The expression of EphA2 was significantly associated with the expression of EphrinA-1 (r = 0.98, P = 0.02). There was no significant correlation between the expressions of EphA2/EphrinA-1 and age, FIGO stage, residual tumour size and histological grade. High levels of both EphA2 and EphrinA-1 protein expression were significantly associated with a shorter overall survival in multivariate analysis (P < 0.05). High levels of EphA2 and EphrinA-1 mRNA and protein were detected in OVCAR3 and SKOV3 cell lines.
There are over-expression of EphA2 and EphrinA-1 in ovarian serous carcinomas and ovarian cancer cell lines. Tumours with higher expression levels of both EphA2 and EphrinA-1 significantly associated with poorer clinical outcome.
探讨EphA2和EphrinA-1在卵巢浆液性癌中的表达及预后意义。
招募95例卵巢浆液性癌患者的肿瘤组织及2种卵巢癌细胞系。采用免疫组织化学方法检测EphA2和EphrinA-1的表达。分析蛋白表达与临床病理特征、患者生存之间的关系。用半定量聚合酶链反应和蛋白质印迹法检测卵巢癌细胞系中EphA2和EphrinA-1的mRNA和蛋白表达。
肿瘤组织中EphA2和EphrinA-1的蛋白表达分别为92.6%（88/95）和97.9%（93/95），而在相邻卵巢组织中分别为40%（8/20）和30%（6/20）（P = 0.000）。EphA2免疫组化染色在肿瘤细胞和血管内皮细胞中均可见，而EphrinA-1主要定位于肿瘤细胞。EphA2的表达与EphrinA-1的表达显著相关（r = 0.98，P = 0.02）。EphA2/EphrinA-1的表达与年龄、国际妇产科联盟（FIGO）分期、残余肿瘤大小及组织学分级之间无显著相关性。多因素分析显示，EphA2和EphrinA-1蛋白表达水平均高与总生存期较短显著相关（P < 0.05）。在OVCAR3和SKOV3细胞系中检测到高水平的EphA2和EphrinA-1 mRNA及蛋白。
EphA2和EphrinA-1在卵巢浆液性癌及卵巢癌细胞系中过表达。EphA2和EphrinA-1表达水平均较高的肿瘤与较差的临床预后显著相关。
