[成都2009年流感流行期间人源样本中甲型（H3N2）流感病毒血凝素基因]

[Hemagglutinin gene of influenza A (H3N2) virus from human samples in the 2009 influenza epidemic in Chengdu].

作者信息

Long Dong-ling, Zhang Xiao-chun, Cao Ran-ran, Deng Qi, Liu Heng-chuan

机构信息

Department of Medical Technology, West China School of Public Health, Sichuan University, Chengdu 610041, China.

出版信息

Sichuan Da Xue Xue Bao Yi Xue Ban. 2011 Mar;42(2):260-3.

PMID:21500568

Abstract

OBJECTIVE

To study the antigenic and genetic characteristics of influenza A (H3N2) virus in the 2009 influenza epidemics in Chengdu.

METHODS

The influenza virus strains were isolated with MDCK cells from 4869 samples taken from the sentinel surveillance in 2009 in Chengdu. Hemagglutination inhibition (HI) and RT-PCR reaction tests were performed to guide the extraction of viral RNA from the culture fluid of the influenza A (H3N2) virus. The hemagglutinin gene was obtained by RT-PCR and sequenced.

RESULTS

The separation rates of swine influenza H1N1, H3N2, H1N1, and B were 25.2%, 7.2%, 4.5%, and 1.5% respectively. The epidemic peaked in summer and autumn. Four amino acids changed in A, B, and D antigenic and receptor binding sites: site160N>K, site174K>R/N, site189K>Q, site277R>Q. Glycosylation sites were inserted to sitel60 or absent at site181 in some isolated strains.

CONCLUSION

Swine influenza H1N1 viruses dominated the 2009 Chengdu epidemic, with H3N2, H1N1, and B strains coexisting. The influenza A(H3N2) viruses had gene variations due to antigenic drift.

摘要

目的

研究2009年成都流感流行期间甲型H3N2流感病毒的抗原性及基因特征。

方法

采用MDCK细胞从2009年成都哨点监测采集的4869份样本中分离流感病毒株。进行血凝抑制（HI）试验及逆转录-聚合酶链反应（RT-PCR）试验，以指导从甲型H3N2流感病毒培养液中提取病毒RNA。通过RT-PCR获得血凝素基因并进行测序。

结果

甲型H1N1、H3N2、H1N1和乙型流感病毒的分离率分别为25.2%、7.2%、4.5%和1.5%。疫情在夏秋季节达到高峰。A、B和D抗原性及受体结合位点有4个氨基酸发生变化：第160位N>K、第174位K>R/N、第189位K>Q、第277位R>Q。部分分离株在第160位插入糖基化位点或第181位无此位点。