Sasaki A, Fuchimoto S, Orita K
First Department of Surgery, Okayama University Medical School, Japan.
Acta Med Okayama. 1990 Dec;44(6):309-14. doi: 10.18926/AMO/30436.
Meth A-fibrosarcoma bearing BALB/c mice were subjected to selected splenic irradiation (2.0-4.0 Gy) on days 7 and 14 of tumor growth. Tumor growth was recorded by serial measurement. Irradiation given on day 7 caused regression of tumor, but irradiation given on day 14 did not show tumor regression. Antitumor activity in the Winn assay was detected in spleen cells 3 days after irradiation, but was not detected 7 days after. The cell surface phenotypes were analyzed on days 3, 7 and 14 of splenic irradiation using monoclonal antibodies (anti-Thy1.2 antibody, anti-Lyt1 antibody, anti-Lyt2 antibody, anti-L3T4 antibody) by flow cytometry. Thy 1.2, Lyt1, and L3T4 cells were increased on day 3 of splenic irradiation, but were not on days 7 and 14. Lyt2-cells did not show increase on days 3, 7 and 14. It was possibly suggested that selected splenic irradiation induced tumor regression was caused by the ability of irradiation to preferentially eliminate suppressor T cells, thereby allowing effector T-cells to become relatively dominant.
携带甲基化纤维肉瘤的BALB/c小鼠在肿瘤生长的第7天和第14天接受了选定的脾脏照射（2.0 - 4.0 Gy）。通过连续测量记录肿瘤生长情况。第7天给予的照射导致肿瘤消退，但第14天给予的照射未显示肿瘤消退。照射后3天在脾细胞中检测到温氏试验中的抗肿瘤活性，但7天后未检测到。在脾脏照射的第3天、第7天和第14天，使用单克隆抗体（抗Thy1.2抗体、抗Lyt1抗体、抗Lyt2抗体、抗L3T4抗体）通过流式细胞术分析细胞表面表型。脾脏照射第3天Thy 1.2、Lyt1和L3T4细胞增加，但第7天和第14天未增加。Lyt2细胞在第3天、第7天和第14天未显示增加。可能提示选定的脾脏照射诱导的肿瘤消退是由于照射优先消除抑制性T细胞的能力，从而使效应性T细胞相对占主导地位所致。
