Masuda E, Shishido T, Fujimoto R, Maeda H
Molecular Medicine Research Laboratories, Yamanouchi Pharmaceutical Co., Ibaraki, Japan.
Immunopharmacology. 1997 Aug;37(1):105-16. doi: 10.1016/s0162-3109(97)00040-4.
We previously reported that tumor eradication was induced by a single injection of neocarzinostatin (NCS) between 1 day and 4 weeks before Meth A transplantation in Balb/c mice via augmenting host-mediated antitumor activity. In order to elucidate the mechanism of this tumor eradication, the cellular components of spleen and regional lymph nodes, tumor infiltrating cells and antitumor effector cells were investigated. Pretreatment with NCS on day -3 caused an increase in the percentage of T-cell subsets, a decrease in the percentage of B-cells, Mac-1+ cells and asialo GM1+ cells and a decrease of the total cell number in the spleen. These changes were observed before but not during the period of tumor regression and were also observed in non-transplanted mice with NCS treatment. In the lymph nodes, while B-cells increased on Meth A transplantation, this was suppressed by NCS pretreatment. Although histological examination of tumor nodules showed the presence of only a few host immune cells in the tumor tissue, the area of necrosis was already extensive on day 7 and expanded thereafter. In vivo depletion of whole T-cells, T-cell subsets or asialo GM1+ cells by antibody treatment suggests that the antitumor effector cells in tumor eradication were Thy1,2+/Lyt2+, and at least some of which also express asialo GM1 antigen and that L3T4+ T-cells were also involved in tumor eradication.
我们先前报道，在Balb/c小鼠中，于甲氨蝶呤A移植前1天至4周单次注射新制癌菌素（NCS），通过增强宿主介导的抗肿瘤活性可诱导肿瘤消除。为阐明这种肿瘤消除的机制，我们对脾脏和区域淋巴结的细胞成分、肿瘤浸润细胞及抗肿瘤效应细胞进行了研究。在第-3天用NCS预处理导致脾脏中T细胞亚群百分比增加，B细胞、Mac-1+细胞和去唾液酸GM1+细胞百分比降低，以及总细胞数减少。这些变化在肿瘤消退期之前而非期间观察到，并且在接受NCS治疗的未移植小鼠中也观察到。在淋巴结中，虽然甲氨蝶呤A移植后B细胞增加，但NCS预处理可抑制这种增加。尽管对肿瘤结节的组织学检查显示肿瘤组织中仅存在少数宿主免疫细胞，但在第7天坏死区域已经广泛，此后还会扩大。通过抗体处理在体内清除全T细胞、T细胞亚群或去唾液酸GM1+细胞表明，肿瘤消除中的抗肿瘤效应细胞是Thy1,2+/Lyt2+，其中至少一些也表达去唾液酸GM1抗原，并且L3T4+ T细胞也参与了肿瘤消除。
