Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Clin Oral Implants Res. 2011 Nov;22(11):1242-6. doi: 10.1111/j.1600-0501.2010.02043.x. Epub 2011 Apr 19.
To mechanically evaluate the effect of transplantation of precultured preosteoblasts derived from autogenic adult mesenchymal stem cells (aMSC) for experimental sinus floor augmentation on primary dental implant stability in comparison with conventional augmentation procedures in rabbits.
After experimental sinus floor augmentation with a synthetic bone substitute, autogenous bone transplantation or osteoblast precursor cells, the primary stability of implants inserted in the edentulous part of the upper jaw of New Zealand White Rabbits was examined. Mechanical evaluation was performed by determination of insertion torque values (Osseocare(™) ), percussion testing (Periotest(™) ), resonance frequency analysis (Osstell(™) and scanning laser Doppler vibrometer) and measurement of extraction forces.
Evaluation of mechanical properties with percussion testing and resonance frequency analysis with Osstell(™) revealed slightly higher primary stability of the stem cell group whereas the scanning laser Doppler vibrometer and measurement of pull-out forces showed no significant difference to the bone substitute group. Transplantation of autogenous bone graft resulted in the highest primary implant stability.
The three examination modalities proved suitable for the determination of primary implant stability. The experimental maxillary sinus floor augmentation with precultured osteoblast precursor cells from autogenic stems cells clearly enhanced the primary stability of implants compared with the unaugmented sinus and lead to comparable primary mechanical properties to bone substitutes in rabbits. In comparison with the autogenous bone graft stability enhancement by stem cell transplantation declined.
通过机械评估自体成体间充质干细胞（aMSC）衍生的预培养前成骨细胞在兔实验性上颌窦底提升中对初级牙种植体稳定性的影响，与常规提升程序进行比较。
在上颌骨无牙区，在使用合成骨替代物、自体骨移植或成骨前体细胞进行实验性上颌窦底提升后，检测植入物的初级稳定性。通过插入扭矩值（Osseocare(™)）、叩诊测试（Periotest(™)）、共振频率分析（Osstell(™)和扫描激光多普勒测振仪）和拔出力测量来进行机械评估。
通过叩诊测试和使用 Osstell(™)进行的共振频率分析评估机械性能，结果显示干细胞组的初级稳定性略高，而扫描激光多普勒测振仪和拔出力测量则显示与骨替代物组无显著差异。自体骨移植物的移植导致初级种植体稳定性最高。
三种检查方式均适合用于确定初级种植体稳定性。与未增强的窦相比，用预培养的自体干细胞来源的成骨前体细胞进行实验性上颌窦底提升明显增强了种植体的初级稳定性，并导致兔初级机械性能与骨替代物相当。与自体骨移植物的稳定性增强相比，干细胞移植的稳定性增强有所下降。
