Department of Microbiology (BK21), School of Medicine, Kyung Hee University, Seoul, Republic of Korea.
Int Immunopharmacol. 2011 Sep;11(9):1271-7. doi: 10.1016/j.intimp.2011.04.007. Epub 2011 Apr 17.
Atopic dermatitis (AD) is one of the most common skin diseases, and its incidence is increasing in industrialized countries. Furthermore, the epicutaneous application of a hapten, such as 2,4-dinitrofluorobenzene (DNFB), evokes an AD-like lesion in NC/Nga mice under specific pathogen-free (SPF) conditions. Rosmarinic acid (RA) is a secondary metabolite that is frequently found in herbs, and has anti-inflammatory, anti-oxidant, and anti-microbial effects. In this study, we studied whether RA is an effective treatment against DNFB-induced AD-like skin lesions in NC/Nga mice. RA at 1 or 5 μM was found to suppress the productions of interferon (IFN)-γ and interleukin (IL)-4 significantly by activated CD4(+) T cells. Furthermore, an intraperitoneal injection of RA at 10 or 50 mg/kg significantly inhibited skin lesion development and ear thickness and total serum IgE level increases in DNFB-treated NC/Nga mice. In addition, intraperitoneal administered RA at 10 or 50 mg/kg significantly inhibited the infiltrations of CD4(+) T, CD8(+) T, and mast cells into DNFB-induced skin lesions in NC/Nga mice. This study suggests that RA suppresses the development of AD-like dermatitis in DNFB-treated NC/Nga mice by reducing IFN-γ and IL-4 production by activated T cells and total serum IgE levels.
特应性皮炎（AD）是最常见的皮肤病之一，在工业化国家其发病率正在增加。此外，在无特定病原体（SPF）条件下，变应原如 2,4-二硝基氟苯（DNFB）经皮应用于 NC/Nga 小鼠，可诱发类似 AD 的病变。迷迭香酸（RA）是一种在草药中经常发现的次级代谢产物，具有抗炎、抗氧化和抗菌作用。在这项研究中，我们研究了 RA 是否是治疗 NC/Nga 小鼠 DNFB 诱导的 AD 样皮肤损伤的有效方法。结果发现，RA 在 1 或 5 μM 时可显著抑制活化的 CD4+T 细胞产生干扰素（IFN）-γ和白细胞介素（IL）-4。此外，腹腔注射 RA 10 或 50mg/kg 可显著抑制 DNFB 处理的 NC/Nga 小鼠的皮肤病变发展和耳朵厚度以及总血清 IgE 水平的增加。此外，腹腔内给予 RA 10 或 50mg/kg 可显著抑制 CD4+T、CD8+T 和肥大细胞浸润到 NC/Nga 小鼠的 DNFB 诱导的皮肤损伤中。本研究表明，RA 通过减少活化 T 细胞产生的 IFN-γ和 IL-4 以及总血清 IgE 水平，抑制 DNFB 处理的 NC/Nga 小鼠 AD 样皮炎的发展。
