Kavun V Ia, Chepkasova A I, Podgurskaia O V, Kovalev N N
Izv Akad Nauk Ser Biol. 2011 Mar-Apr(2):220-6.
The effects of environmental conditions on cholinesterase activity and kinetic parameters of substrate hydrolysis in the hemolymph of the mussel Crenomytilus grayanus were studied. Under seasonal upwellings, the cholinergic system efficiency is provided for by a wide range of efficient concentrations of the substrate, i.e., under such conditions the mussels at the molecular level have a quantitative adaptation strategy of the enzyme. In mussels from the stationary upwelling zone (at a steady low temperature of water) for efficiency of the cholinergic system, the quantitative strategy of enzyme adaptation is realized. In mussels from a highly contaminated site, irreversible damages to the cholinergic process were observed. The affinity of the substrate to the enzyme is highly informative and an appropriate biomarker for the load level and the adaptation capacity of the organism. The affinity of the substrate to the enzyme is recommended as a new biomarker.
研究了环境条件对贻贝（Crenomytilus grayanus）血淋巴中胆碱酯酶活性及底物水解动力学参数的影响。在季节性上升流期间，底物的广泛有效浓度确保了胆碱能系统的效率，即在这种条件下，贻贝在分子水平上具有酶的定量适应策略。在来自稳定上升流区域（水温稳定较低）的贻贝中，实现了酶适应的定量策略以确保胆碱能系统的效率。在来自高污染位点的贻贝中，观察到胆碱能过程的不可逆损伤。底物与酶的亲和力具有高度信息性，是反映生物体负荷水平和适应能力的合适生物标志物。建议将底物与酶的亲和力作为一种新的生物标志物。
