Ojha Shreesh, Nandave Mukesh, Kumaria Santosh, Arya Dharamvir S
Cardiovascular Laboratory, Department of Pharmacology, All India Institute of Medical Sciences, New Delhi 110 029, India.
Indian J Exp Biol. 2010 Sep;48(9):918-24.
To evaluate the cardioprotective potential of Inula racemosa in myocardial ischemic-reperfusion injury, Wistar male albino rats were randomly divided into four groups. The group I and II animals were administered saline orally {(sham, ischemia- reperfusion (I-R) control group)} and animals of group III and group IV received I. racemosa extract (100 mg/kg) for 30 days. On the 30th day, animals of I-R control and I. racemosa treated groups were underwent 45 min of ligation of left anterior descending coronary artery and were thereafter re-perfused for 60 min. In the I-R control group, a significant decrease of mean arterial pressure (MAP), heart rate (HR), contractility, (+)LVdP/dt and relaxation, (-)LVdP/dt and an increase of left ventricular end diastolic pressure (LVEDP) were observed. Subsequent to haemodynamic impairment and left ventricular contractile dysfunction, a significant decline was observed in endogenous myocardial antioxidants; superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and reduced glutathione (GSH). Increased lipid peroxidation characterized by malonaldialdehyde (MDA) formation along with depletion of cardiomyocytes specific enzymes, creatine phosphokinase-MB (CK-MB) isoenzyme and lactate dehydrogenase (LDH) in I-R control group compared to sham group revealed I-R injury of heart. However, treatment with I. racemosa significantly restored the myocardial antioxidant status evidenced by increased SOD, CAT, GPx and GSH and prevented leakage of cardio-specific enzymes; CK-MB and LDH and favorably modulated the altered MAP, HR, (+)LVdP/dt, (-)LVdP/dt and LVEDP as compared to I-R control. Furthermore, I-R induced lipid peroxidation was significantly inhibited by I. racemosa treatment. These beneficial cardioprotective effects translated into significant improvement in cardiac function. In conclusion, our study has demonstrated that the cardioprotective effect of I. racemosa likely resulted to improved antioxidant status, haemodynamic and left ventricular contractile function subsequent to suppression of oxidative stress.
为了评估旋覆花对心肌缺血再灌注损伤的心脏保护潜力，将雄性Wistar白化大鼠随机分为四组。第I组和第II组动物口服生理盐水（假手术组、缺血再灌注（I-R）对照组），第III组和第IV组动物接受旋覆花提取物（100 mg/kg），持续30天。在第30天，I-R对照组和旋覆花治疗组的动物进行左冠状动脉前降支结扎45分钟，然后再灌注60分钟。在I-R对照组中，观察到平均动脉压（MAP）、心率（HR）、收缩力、（+）左室压力变化率（LVdP/dt）和舒张、（-）LVdP/dt显著降低，左心室舒张末期压力（LVEDP）升高。在血流动力学损害和左心室收缩功能障碍之后，观察到内源性心肌抗氧化剂超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）和还原型谷胱甘肽（GSH）显著下降。与假手术组相比，I-R对照组中以丙二醛（MDA）形成为特征的脂质过氧化增加，同时心肌特异性酶肌酸磷酸激酶-MB（CK-MB）同工酶和乳酸脱氢酶（LDH）耗竭，揭示了心脏的I-R损伤。然而，旋覆花治疗显著恢复了心肌抗氧化状态，表现为SOD、CAT、GPx和GSH增加，并防止了心脏特异性酶CK-MB和LDH的泄漏，与I-R对照组相比，有利地调节了改变的MAP、HR、（+）LVdP/dt、（-）LVdP/dt和LVEDP。此外，旋覆花治疗显著抑制了I-R诱导的脂质过氧化。这些有益的心脏保护作用转化为心脏功能的显著改善。总之，我们的研究表明，旋覆花的心脏保护作用可能是由于氧化应激受到抑制后抗氧化状态、血流动力学和左心室收缩功能得到改善。
