Sayyed R Z, Gangurde N S
PG Department of Microbiology, S. I. Patil Arts, G. B. Patel Science and S.T.S.K.V.S. Commerce College, Shahada, Dist. Nandurbar 425409, India.
Indian J Exp Biol. 2010 Sep;48(9):942-7.
Pseudomonas sp. RZS1 was isolated from distillery effluent and identified based on phenotypic characters and 16s rRNA sequencing. It accumulated optimum amount (703.79 microg/mg of biomass) of poly-beta-hydroxybutyrate (PHB) under aerobic process of fermentation and 75 microg/mg of biomass under the anaerobic process of fermentation. Aerobic fermentation yielded 9.3-fold more PHB than semi-aerobic fermentation. Acetone alcohol method proved to be the best suitable recovery method as it gave 703.79 microg PHB per mg of biomass with a percentage recovery yield of 70.37. It started to accumulate PHB at the end of lag phase (from 6 h of incubation). Optimum amount of PHB (20 microg/ml) was reported during early stationary phase (30 h of incubation). Extracted PHB showed two peaks, minor one at 248 nm and major one at 365 nm. IR spectra revealed the presence of functional groups characteristics of PHB.
假单胞菌属RZS1从酿酒厂废水分离得到，并基于表型特征和16S rRNA测序进行鉴定。在有氧发酵过程中，它积累了最佳量（703.79微克/毫克生物量）的聚-β-羟基丁酸酯（PHB），在厌氧发酵过程中积累了75微克/毫克生物量。有氧发酵产生的PHB比半好氧发酵多9.3倍。丙酮酒精法被证明是最合适的回收方法，因为它每毫克生物量可得到703.79微克PHB，回收率为70.37%。它在延滞期末期（培养6小时后）开始积累PHB。在早期稳定期（培养30小时）报告了最佳量的PHB（20微克/毫升）。提取的PHB显示出两个峰，较小的峰在248纳米处，较大的峰在365纳米处。红外光谱揭示了PHB特征官能团的存在。
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