Sayyed R Z, Shaikh S S, Wani S J, Rehman Md Tabish, Al Ajmi Mohammad F, Haque Shafiul, El Enshasy Hesham Ali
Department of Microbiology, PSGVP Mandal's, Arts, Science and Commerce College, SHAHADA, Maharashtra 425 409, India.
Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11564, Saudi Arabia.
Molecules. 2021 Apr 22;26(9):2443. doi: 10.3390/molecules26092443.
The present study was aimed to evaluate the suitability of agro-wastes and crude vegetable oils for the cost-effective production of poly-β-hydroxybutyrate (PHB), to evaluate growth kinetics and PHB production in RZS4 and sp. RZS1 with these carbon substrates and to study the biodegradation of PHB accumulated by these cultures. RZS4 and sp. RZS1 accumulates higher amounts of PHB corn (79.90% of dry cell mass) and rice straw (66.22% of dry cell mass) medium respectively. The kinetic model suggests that the sp. RZS1 follows the Monod model more closely than RZS4. Both the cultures degrade their PHB extract under the influence of PHB depolymerase. Corn waste and rice straw appear as the best and cost-effective substrates for the sustainable production of PHB from RZS4 and sp. RZS1. The biopolymer accumulated by these organisms is biodegradable in nature. The agro-wastes and crude vegetable oils are good and low-cost sources of nutrients for the growth and production of PHB and other metabolites. Their use would lower the production cost of PHB and the low-cost production will reduce the sailing price of PHB-based products. This would promote the large-scale commercialization and popularization of PHB as an ecofriendly bioplastic/biopolymer.
本研究旨在评估农业废弃物和粗植物油用于经济高效生产聚-β-羟基丁酸酯（PHB）的适用性，评估RZS4和RZS1菌株利用这些碳源底物时的生长动力学和PHB产量，并研究这些培养物积累的PHB的生物降解情况。RZS4和RZS1菌株分别在玉米（占干细胞质量的79.90%）和稻草（占干细胞质量的66.22%）培养基中积累了更高量的PHB。动力学模型表明，RZS1菌株比RZS4菌株更符合莫诺德模型。在PHB解聚酶的作用下，两种培养物都能降解其PHB提取物。玉米废弃物和稻草似乎是RZS4和RZS1菌株可持续生产PHB的最佳且经济高效的底物。这些生物体积累的生物聚合物在自然界中是可生物降解的。农业废弃物和粗植物油是用于PHB及其他代谢产物生长和生产的优质且低成本的营养源。它们的使用将降低PHB的生产成本，而低成本生产将降低基于PHB产品的售价。这将促进PHB作为一种环保型生物塑料/生物聚合物的大规模商业化和推广。
