Novel M, Huang X F, Novel G
Laboratoire de Génétique Microbienne, IRBA Université de Caen, France.
FEMS Microbiol Lett. 1990 Nov;60(3):309-14. doi: 10.1016/0378-1097(90)90323-i.
A recA-like gene was isolated from a gene library of Lactococcus lactis subsp. lactis by intergeneric complementation of an E. coli recA mutant. A plasmid was obtained which fully complemented the RecA response to DNA damaging agents and UV inducibility of prophage, but not P1 plating efficiency in an E. coli recA mutant. The cloned DNA fragment also partially complemented the rec mutation in Lc. lactis MMS36. Hybridization studies showed that there was no detectable sequence homology between the recA gene of E. coli and Lc. lactis subsp. lactis chromosomal DNA.
通过大肠杆菌recA突变体的属间互补，从乳酸乳球菌乳酸亚种的基因文库中分离出一个类recA基因。获得了一个质粒，该质粒完全互补了RecA对DNA损伤剂的反应以及原噬菌体的紫外线诱导性，但在大肠杆菌recA突变体中不能互补P1的平板效率。克隆的DNA片段也部分互补了乳酸乳球菌MMS36中的rec突变。杂交研究表明，大肠杆菌的recA基因与乳酸乳球菌乳酸亚种染色体DNA之间没有可检测到的序列同源性。
