Aminov R I, Nagamine T, Ogata K, Sugiura M, Tajima K, Benno Y
STAFF-Institute, Ibaraki, Japan.
FEMS Microbiol Lett. 1996 Oct 15;144(1):53-9. doi: 10.1111/j.1574-6968.1996.tb08508.x.
Degenerate PCR primers based on conserved RecA protein regions were used to amplify a portion of recA [corrected] from Prevotella ruminicola strain 23, which was used as a probe to isolate the full-length recA gene from the P. ruminicola genomic library. The P. ruminicola recA gene encoded a protein of 340 amino acids with a molecular mass of 36.81 kDa, P. ruminicola RecA was highly similar to other RecA proteins and most closely resembled that of Bacteroides fragilis (75% identity). It alleviated the methyl methanesulfonate and mitomycin C sensitivities of Escherichia coli recA mutants, but did not restore the resistance to UV-light irradiation. Mitomycin C treatment of otherwise isogenic E. coli strains showed a higher level of prophage induction in a recA harboring lysogen.
