Shawar R, Cooper B H
Department of Obstetrics and Gynecology, University of Texas Medical School, Houston 77225.
Photochem Photobiol. 1990 Oct;52(4):825-30. doi: 10.1111/j.1751-1097.1990.tb08688.x.
Hematoporphyrin derivative (HpD) is widely used in photoradiation therapy of tumors and other diseases, and has been shown to affect the viability of gram positive bacteria. This investigation assessed the efficiency of binding of HpD to Bacillus subtilis and Streptococcus faecalis when HpD-treated organisms were exposed to red light. Kinetic studies indicated that the amount of HpD bound increased with increasing external concentration of HpD until saturation of binding sites was reached. S. faecalis had a higher affinity for HpD and was more susceptible to photoinactivation than B. subtilis. The data from this study suggest that differences in susceptibility of microorganisms to photoinactivation are directly related to the affinity of each strain for HpD.
血卟啉衍生物（HpD）广泛应用于肿瘤及其他疾病的光辐射治疗，且已证明其会影响革兰氏阳性菌的生存能力。本研究评估了经HpD处理的生物体暴露于红光下时，HpD与枯草芽孢杆菌和粪肠球菌的结合效率。动力学研究表明，结合的HpD量随着外部HpD浓度的增加而增加，直至结合位点饱和。粪肠球菌对HpD的亲和力更高，比枯草芽孢杆菌更易受到光灭活作用的影响。本研究数据表明，微生物对光灭活作用敏感性的差异与各菌株对HpD的亲和力直接相关。
