Selman S H, Kreimer-Birnbaum M, Goldblatt P J, Anderson T S, Keck R W, Britton S L
Cancer Res. 1985 Dec;45(12 Pt 1):6425-7.
Experiments were performed to determine the effect of hematoporphyrin derivative (HPD) photodynamic therapy on blood flow to normal rat intestine. A segment of rat jejunum was exposed to red (greater than 590 nm) light (200 mW/cm2) 24 h after the i.v. administration of HPD. Blood flow to the light exposed segment was determined using the radioactive microsphere technique while blood flow to an adjacent light shielded segment of intestine served as an internal control. Animals were divided into six groups of six each: Group I, no HPD, no light; Group II, light, no HPD; Group III, HPD (20 micrograms/g body weight), no light; and Group IV, HPD (20 micrograms/g body weight), light. Blood flow in these four groups was determined 10 min after completion of a 30-min exposure to light. Only in Group IV was there a statistically significant decrease (P less than 0.005) in blood flow to the segment treated with HPD and light. In Groups V [HPD (20 micrograms/g body weight), light] and VI [HPD (10 micrograms/g body weight), light] blood flows were determined 24 h after exposure to light. In both of these groups there was also a significant (P less than 0.05) decrease in blood flow in the segment treated with HPD and light. This study demonstrates that normal intestinal blood flow can be disrupted by HPD photodynamic therapy.
进行实验以确定血卟啉衍生物（HPD）光动力疗法对正常大鼠肠道血流的影响。在静脉注射HPD 24小时后，将一段大鼠空肠暴露于红色（大于590nm）光（200mW/cm²）下。使用放射性微球技术测定暴露于光的肠段的血流，而相邻的避光肠段的血流作为内部对照。将动物分为六组，每组六只：第一组，不注射HPD，不照射光；第二组，照射光，不注射HPD；第三组，注射HPD（20微克/克体重），不照射光；第四组，注射HPD（20微克/克体重），照射光。在30分钟光照结束后10分钟测定这四组的血流。仅在第四组中，接受HPD和光照处理的肠段的血流有统计学显著下降（P小于0.005）。在第五组[注射HPD（20微克/克体重），照射光]和第六组[注射HPD（10微克/克体重），照射光]中，在光照24小时后测定血流。在这两组中，接受HPD和光照处理的肠段的血流也有显著（P小于0.05）下降。本研究表明，HPD光动力疗法可破坏正常肠道血流。
