Institute of Legal Medicine, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany.
J Anal Toxicol. 2011 May;35(4):248-53. doi: 10.1093/anatox/35.4.248.
A sensitive method for identifying and quantifying brucine by means of liquid chromatography-tandem mass spectrometry is presented in this article. Based on a solid-phase extraction for human serum, the validation indicated limits of detection and quantification of 0.12 and 0.23 ng/mL, respectively. In one case of lethal suicidal brucine monointoxication, brucine concentrations of 1.51 μg/mL, 1.69 μg/mL, 9.94 μg/mL, 16.4 μg/g, 0.99 μg/g, 0.75 μg/g, and 1.95 mg/g were determined in femoral blood, urine, bile collected from the gallbladder, liver tissue, cerebellum, cerebrum, and stomach contents, respectively.
本文提出了一种利用液相色谱-串联质谱法鉴定和定量士的宁的灵敏方法。通过固相萃取对人血清进行处理，验证结果表明检测限和定量限分别为 0.12ng/mL 和 0.23ng/mL。在一例致死性自杀性士的宁单中毒案例中，分别在股血、尿液、从胆囊采集的胆汁、肝组织、小脑、大脑和胃内容物中检测到士的宁浓度为 1.51μg/mL、1.69μg/mL、9.94μg/mL、16.4μg/g、0.99μg/g、0.75μg/g 和 1.95mg/g。
