Francke B
Biochemistry. 1978 Dec 12;17(25):5494-9. doi: 10.1021/bi00618a026.
The effect of polyamines on cell-free DNA synthesis of herpes simplex virus DNA in two different systems is investigated. Purified nuclei from infected cells are devoid of measurable amounts of putrescine, spermidine, and spermine, while an unfractionated lysate contains the polyamines at close to their respective cellular concentrations. Spermine, 0.3 mM, and 0.5 mM spermidine, when added to the nuclear system, decrease the extent of viral DNA synthesis to the level found in the lysate system, the size of the cell-free viral DNA product is increased, and a specific inhibition of repair-type DNA synthesis is observed. These effects of the polyamines occur only in the presence of ATP and not the other three ribonucleoside triphosphates.
研究了多胺在两种不同体系中对单纯疱疹病毒DNA无细胞合成的影响。感染细胞的纯化细胞核中不含可测量量的腐胺、亚精胺和精胺，而未分级的裂解物中所含的多胺浓度接近其各自的细胞浓度。当向细胞核体系中添加0.3 mM的精胺和0.5 mM的亚精胺时，病毒DNA合成的程度降低至裂解物体系中的水平，无细胞病毒DNA产物的大小增加，并且观察到对修复型DNA合成的特异性抑制。多胺的这些作用仅在ATP存在时发生，而在其他三种核糖核苷三磷酸存在时不发生。
