Harker C L, Ellis T H, Coen E S
John Innes Institute, Norwich, United Kingdom.
Plant Cell. 1990 Mar;2(3):185-94. doi: 10.1105/tpc.2.3.185.
Chalcone synthase (CHS) is a key enzyme in the biosynthesis of diverse flavonoids involved in disease resistance, nodulation, and pigmentation in pea. We describe a multigene family encoding CHS and the effects of two regulatory loci, a and a2, on the pattern of expression of three of its member genes. Two of the genes, CHS1 and CHS3, are expressed in both petal and root tissue, whereas expression of a third gene, CHS2, is detected only in roots. The products encoded by the a and a2 loci are required for the expression of the CHS1 gene and for wild-type levels of expression of the CHS3 gene in petal tissue. In root tissue, all three CHS genes are expressed and induced by CuCl2 regardless of the genotype at the a and a2 loci. These results show that the various members of the CHS multigene family interact in diverse ways with multiple genetic signals in the plant, providing a basis for the differential expression of these genes. Spatially specific genetic regulation of distinct members of a multigene family has been clearly demonstrated.
查尔酮合酶（CHS）是豌豆中参与抗病性、结瘤和色素沉着的多种类黄酮生物合成的关键酶。我们描述了一个编码CHS的多基因家族，以及两个调控位点a和a2对其三个成员基因表达模式的影响。其中两个基因CHS1和CHS3在花瓣和根组织中均有表达，而第三个基因CHS2仅在根中检测到表达。a和a2位点编码的产物是CHS1基因表达以及花瓣组织中CHS3基因野生型表达水平所必需的。在根组织中，无论a和a2位点的基因型如何，所有三个CHS基因均表达并受CuCl2诱导。这些结果表明，CHS多基因家族的各个成员以多种方式与植物中的多种遗传信号相互作用，为这些基因的差异表达提供了基础。多基因家族不同成员的空间特异性遗传调控已得到明确证明。
