McLean B G, Eubanks S, Meagher R B
Department of Genetics, University of Georgia, Athens 30602.
Plant Cell. 1990 Apr;2(4):335-44. doi: 10.1105/tpc.2.4.335.
It has been proposed that the evolution of distinct classes of genes encoding the kappa-, lambda-, and mu-actins in soybean is the result of an ancient divergence in patterns of actin gene expression. In this study, antisera against a family of synthetic actin peptides from a divergent region within the predicted actin polypeptide sequences have been used to explore the differential expression of plant actins. Antiserum elicited against a 16-residue synthetic lambda-actin peptide SAc4:257 reacted with a 46-kilodalton protein in soybean extracts, showed specificity for the lambda-peptide over the divergent kappa- and mu-actin peptides in enzyme-linked immunosorbent assays, and reacted strongly and preferentially with root protoderm in apical roots and in lateral root primordia. Antiserum elicited against the synthetic kappa-actin peptide SAc1:257 reacted with 46-kilodalton protein on protein gel blots, showed partial specificity toward the immunogenic kappa-peptide over the divergent lambda- and mu-peptides, and reacted strongly with all root tissues with the exception of root cap. These data support the hypothesis that ancient classes of plant actin genes may have been preserved because of their role in developmentally controlled differences in tissue-specific actin expression and/or function. The possibility that other diverse actin classes have unique patterns of regulation is discussed.
有人提出，大豆中编码κ-、λ-和μ-肌动蛋白的不同类别基因的进化，是肌动蛋白基因表达模式古老分化的结果。在本研究中，针对预测的肌动蛋白多肽序列中一个不同区域的合成肌动蛋白肽家族的抗血清，已被用于探索植物肌动蛋白的差异表达。针对16个残基的合成λ-肌动蛋白肽SAc4:257产生的抗血清，与大豆提取物中的一种46千道尔顿的蛋白质发生反应，在酶联免疫吸附测定中对λ-肽比对不同的κ-和μ-肌动蛋白肽具有特异性，并且在根尖和侧根原基中与根原表皮强烈且优先反应。针对合成κ-肌动蛋白肽SAc1:257产生的抗血清，在蛋白质凝胶印迹上与46千道尔顿的蛋白质发生反应，对免疫原性κ-肽比对不同的λ-和μ-肽具有部分特异性，并且除根冠外与所有根组织强烈反应。这些数据支持了这样一种假设，即古老的植物肌动蛋白基因类别可能因其在组织特异性肌动蛋白表达和/或功能的发育控制差异中的作用而得以保留。本文还讨论了其他不同肌动蛋白类别具有独特调控模式的可能性。
