Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, High Council for Scientific Research, Granada, Spain.
PLoS One. 2012;7(2):e30878. doi: 10.1371/journal.pone.0030878. Epub 2012 Feb 14.
Profilin, a multigene family involved in actin dynamics, is a multiple partners-interacting protein, as regard of the presence of at least of three binding domains encompassing actin, phosphoinositide lipids, and poly-L-proline interacting patches. In addition, pollen profilins are important allergens in several species like Olea europaea L. (Ole e 2), Betula pendula (Bet v 2), Phleum pratense (Phl p 12), Zea mays (Zea m 12) and Corylus avellana (Cor a 2). In spite of the biological and clinical importance of these molecules, variability in pollen profilin sequences has been poorly pointed out up until now. In this work, a relatively high number of pollen profilin sequences have been cloned, with the aim of carrying out an extensive characterization of their polymorphism among 24 olive cultivars and the above mentioned plant species. Our results indicate a high level of variability in the sequences analyzed. Quantitative intra-specific/varietal polymorphism was higher in comparison to inter-specific/cultivars comparisons. Multi-optional posttranslational modifications, e.g. phosphorylation sites, physicochemical properties, and partners-interacting functional residues have been shown to be affected by profilin polymorphism. As a result of this variability, profilins yielded a clear taxonomic separation between the five plant species. Profilin family multifunctionality might be inferred by natural variation through profilin isovariants generated among olive germplasm, as a result of polymorphism. The high variability might result in both differential profilin properties and differences in the regulation of the interaction with natural partners, affecting the mechanisms underlying the transmission of signals throughout signaling pathways in response to different stress environments. Moreover, elucidating the effect of profilin polymorphism in adaptive responses like actin dynamics, and cellular behavior, represents an exciting research goal for the future.
丝状肌动蛋白结合蛋白（profilin）是一个涉及肌动蛋白动态变化的多基因家族，它是一种与多个伴侣相互作用的蛋白，至少存在三个结合结构域，包括肌动蛋白、磷酸肌醇脂和多聚 L-脯氨酸结合位点。此外，花粉丝动蛋白是多种植物的重要过敏原，如油橄榄（Olea europaea L.，Ole e 2）、欧洲山杨（Betula pendula，Bet v 2）、梯牧草（Phleum pratense，Phl p 12）、玉米（Zea mays，Zea m 12）和榛子（Corylus avellana，Cor a 2）。尽管这些分子具有重要的生物学和临床意义，但花粉丝动蛋白序列的变异性直到现在才得到充分的研究。在这项工作中，我们克隆了相对较多的花粉丝动蛋白序列，目的是对 24 个油橄榄品种和上述植物物种的花粉丝动蛋白序列进行广泛的多态性分析。我们的研究结果表明，所分析的序列具有高度的变异性。与种间/品种间的比较相比，种内/品种内的多态性更高。多选项翻译后修饰，如磷酸化位点、理化性质和相互作用功能残基，已被证明受丝动蛋白多态性的影响。由于这种变异性，5 种植物物种之间产生了明显的分类分离。丝动蛋白家族的多功能性可以通过在橄榄种质中产生的丝动蛋白同工型的自然变异来推断，这是多态性的结果。高变异性可能导致丝动蛋白特性的差异和与天然伴侣相互作用的调节差异，从而影响信号转导途径中信号传递的机制，以应对不同的应激环境。此外，阐明丝动蛋白多态性对肌动蛋白动态变化和细胞行为等适应性反应的影响，是未来一个令人兴奋的研究目标。
