Exp Dermatol. 2011 Aug;20(8):679-81. doi: 10.1111/j.1600-0625.2011.01281.x. Epub 2011 Apr 27.
Interactions between epithelial and dermal cells are essential for hair follicle morphogenesis and maintenance. In experimental trials of hair regeneration, isolated dermal cells have been shown to possess hair-inducing capacity. However, dermal cells lose this potential immediately after cultivation. Sphere-forming multipotent cells derived from the dermis possess hair-inducing capacity. These previous findings raise the question of whether hair-inducing capacity depends on the identity as dermal cells or the process of sphere formation. To address this issue, we compared the in vitro and in vivo characteristics of two-dimensionally cultured or thereafter sphere formation-induced dermal and lung mesenchymal cells. We show that sphere-forming mesenchymal cells exhibited higher expression of Wnt signalling genes. Sphere-forming cells but not two-dimensionally cultured cells possessed in vivo hair-inducing capacity. These data suggest that various mesenchymal cells attain hair-inducing capacity through the process of sphere formation.
上皮细胞和真皮细胞之间的相互作用对于毛囊形态发生和维持至关重要。在毛发再生的实验研究中，已经证明分离的真皮细胞具有诱导毛发的能力。然而，真皮细胞在培养后立即失去这种潜能。源自真皮的球体形成多能细胞具有诱导毛发的能力。这些先前的发现提出了一个问题，即诱导毛发的能力是否取决于真皮细胞的身份或球体形成的过程。为了解决这个问题，我们比较了二维培养或随后球体形成诱导的真皮和肺间充质细胞的体外和体内特征。我们表明，球体形成的间充质细胞表现出更高水平的 Wnt 信号基因表达。球体形成的细胞而非二维培养的细胞具有体内诱导毛发的能力。这些数据表明，各种间充质细胞通过球体形成过程获得诱导毛发的能力。
