Bioscience Research Institute, Amorepacific Corporation R&D Center, Yongin-si, Republic of Korea.
Stem Cells Dev. 2013 Apr 15;22(8):1264-74. doi: 10.1089/scd.2012.0244. Epub 2013 Jan 22.
Adult stem cells from the dermis would be an attractive cell source for therapeutic purposes as well as studying the process of skin aging. Several studies have reported that human dermal stem/progenitor cells (hDSPCs) with multipotent properties exist within the dermis of adult human skin. However, these cells have not been well characterized, because methods for their isolation or enrichment have not yet been optimized. In the present study, we enriched high side scatter (SSC(high))-hDSPCs from normal human dermal fibroblasts using a structural characteristic, intracellular granularity, as a sorting parameter. The SSC(high)-hDSPCs had high in vitro proliferation properties and expressed high levels of SOX2 and S100B, similar to previously identified mouse SOX2+ hair follicle dermal stem cells. The SSC(high)-hDSPCs could differentiate into not only mesodermal cell types, for example, adipocytes, chondrocytes, and osteoblasts, but also neuroectodermal cell types, such as neural cells. In addition, the SSC(high)-hDSPCs exhibited no significant differences in the expression of nestin, vimentin, SNAI2, TWIST1, versican, and CORIN compared with non-hDSPCs. These cells are therefore different from the previously identified multipotent fibroblasts and skin-derived progenitors. In this study, we suggest that hDSPCs can be enriched by using characteristic of their high intracellular granularity, and these SSC(high)-hDSPCs exhibit high in vitro proliferation and differentiation potentials.
真皮中的成体干细胞作为一种治疗目的的有吸引力的细胞来源，以及研究皮肤衰老过程。有几项研究报道，具有多能性的人真皮干/祖细胞（hDSPC）存在于成人皮肤的真皮中。然而，这些细胞尚未得到很好的表征，因为尚未优化其分离或富集的方法。在本研究中，我们使用细胞内颗粒度作为分选参数，从正常人真皮成纤维细胞中富集高侧向散射（SSC(high)）-hDSPC。SSC(high)-hDSPC 具有高体外增殖特性，并表达高水平的 SOX2 和 S100B，类似于先前鉴定的鼠 SOX2+毛囊真皮干细胞。SSC(high)-hDSPC 不仅可以分化为中胚层细胞类型，例如脂肪细胞、软骨细胞和成骨细胞，还可以分化为神经外胚层细胞类型，如神经细胞。此外，与非 hDSPC 相比，SSC(high)-hDSPC 在巢蛋白、波形蛋白、SNAI2、TWIST1、versican 和 CORIN 的表达上没有显著差异。因此，这些细胞与先前鉴定的多能成纤维细胞和皮肤来源的祖细胞不同。在这项研究中，我们提出可以使用其高细胞内颗粒度的特征来富集 hDSPC，并且这些 SSC(high)-hDSPC 表现出高的体外增殖和分化潜能。
