Research and Biotechnology Division, St. Luke's Medical Centre, Quezon City, Philippines.
Clin Microbiol Infect. 2011 May;17(5):776-9. doi: 10.1111/j.1469-0691.2010.03369.x. Epub 2010 Nov 2.
DNA sequence-based identification of pathogens from ocular samples of patients with clinically suspected eye infections was accomplished using 16S and internal transcribed spacer (ITS) ribosomal RNA gene sequence analysis. PCR was positive for 24 of 99 samples tested. Both culture and 16S rDNA sequence analysis identified Pseudomonas aeruginosa, streptococci and Enterobacteriaceae. Isolates misidentified as Burkholderia cepacia by biochemical tests were identified as Ralstonia mannitolilytica by 16S rDNA sequence analysis. Sequence analysis identified the following microorganisms from 19 culture-negative samples: Haemophilus influenzae, Sphingomonas sp., Klebsiella pneumoniae, Staphylococcus haemolyticus, Morganella morganii, Mycobacterium sp., Chryseobacterium sp., Pseudomonas saccharophila (Xanthomonas) and the fungus, Phaeoacremonium inflatipes.
采用 16S 和内部转录间隔区（ITS）核糖体 RNA 基因序列分析，从临床疑似眼部感染患者的眼部样本中基于 DNA 序列鉴定病原体。99 个测试样本中，24 个 PCR 呈阳性。培养和 16S rDNA 序列分析均鉴定出铜绿假单胞菌、链球菌和肠杆菌科。通过生化试验错误鉴定为洋葱伯克霍尔德菌的分离物，通过 16S rDNA 序列分析鉴定为粘质沙雷氏菌。序列分析从 19 个培养阴性样本中鉴定出以下微生物：流感嗜血杆菌、鞘氨醇单胞菌、肺炎克雷伯菌、溶血葡萄球菌、摩氏摩根菌、分枝杆菌、黄单胞菌和真菌，膨胀嗜热丝孢菌。
