特应性角结膜炎患者与健康个体的结膜微生物组分析
Analysis of the Conjunctival Microbiome in Patients with Atopic Keratoconjunctivitis and Healthy Individuals.
作者信息
Hur Min Seok, Lee Ji Su, Jang Minsu, Shin Hyun Jin, Lee Yang Won
机构信息
Department of Dermatology, Konkuk University School of Medicine, Seoul, Korea.
Department of Ophthalmology, Konkuk University School of Medicine, Seoul, Korea.
出版信息
Ann Dermatol. 2021 Apr;33(2):163-169. doi: 10.5021/ad.2021.33.2.163. Epub 2021 Mar 8.
BACKGROUND
Atopic dermatitis (AD) has been clarified that imbalance of bacterial and fungal communities in the skin and gut play key roles in immunologic dysfunction. Atopic keratoconjunctivitis (AKC), one of severe ophthalmic manifestation of AD, could be related with dysbiosis as same as AD.
OBJECTIVE
In this case-control study, the roles of conjunctival microbial communities in AKC were evaluated by a comparative analysis with healthy controls (HCs).
METHODS
16S rRNA sequencing was used to construct libraries of compositional information for a total of 30 volunteers including 20 patients with AKC and 10 HCs.
RESULTS
In the results, variation in the conjunctival taxonomic composition was higher in patients with AKC than in the HC group. In an analysis of relative abundance at the genus level, some taxa significantly differed between groups, including , , , , , and (<0.05). Beta diversity was significantly higher in patients with AKC than in HCs (PERMANOVA, =0.004).
CONCLUSION
The results indicated that the diversity and composition of the microbiome differs between patients with AKC and HCs.
背景
特应性皮炎（AD）已明确，皮肤和肠道中细菌和真菌群落的失衡在免疫功能障碍中起关键作用。特应性角结膜炎（AKC）是AD的严重眼部表现之一，可能与AD一样与微生物群落失调有关。
目的
在本病例对照研究中，通过与健康对照（HCs）进行比较分析，评估结膜微生物群落在AKC中的作用。
方法
采用16S rRNA测序为总共30名志愿者构建组成信息文库，其中包括20例AKC患者和10名HCs。
结果
结果显示，AKC患者结膜分类组成的变异高于HC组。在属水平的相对丰度分析中，两组之间一些分类群存在显著差异，包括 、 、 、 、 和 （<0.05）。AKC患者的β多样性显著高于HCs（PERMANOVA， =0.004）。
结论
结果表明，AKC患者和HCs之间微生物组的多样性和组成存在差异。
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