de Oliveira D E, Seurinck J, Inzé D, Van Montagu M, Botterman J
Plant Genetic Systems, N.V., Gent, Belgium.
Plant Cell. 1990 May;2(5):427-36. doi: 10.1105/tpc.2.5.427.
Five cDNA clones coding for glycine-rich proteins in Arabidopsis thaliana were isolated. The corresponding genes are present in the genome as single copies. The derived protein sequences contain highly repetitive glycine-rich motifs. There is, however, little homology among them, nor with previously described glycine-rich proteins from other species. All five genes are expressed in leaves and stems of 6-week-old plants but show different patterns of expression in other organ systems. Analysis of the effect of different external stimuli on the expression pattern showed that, in most cases, the transcript levels were moderately but selectively affected. With flooding stress, the accumulated level of the transcript from one of the genes was remarkably increased.
分离出了五个编码拟南芥富含甘氨酸蛋白的cDNA克隆。相应的基因在基因组中以单拷贝形式存在。推导的蛋白质序列包含高度重复的富含甘氨酸基序。然而,它们之间以及与其他物种先前描述的富含甘氨酸蛋白之间几乎没有同源性。所有五个基因在6周龄植物的叶和茎中表达,但在其他器官系统中表现出不同的表达模式。对不同外部刺激对表达模式的影响分析表明,在大多数情况下,转录水平受到适度但选择性的影响。在淹水胁迫下,其中一个基因的转录本积累水平显著增加。