van Nocker S, Vierstra R D
Department of Horticulture, University of Wisconsin-Madison 53706.
Plant Mol Biol. 1993 Feb;21(4):695-9. doi: 10.1007/BF00014552.
Two related DNA sequences encoding small (-17kDa) glycine-rich proteins (GRPs) have been isolated from an Arabidopsis thaliana cDNA library. AtGRP7 and AtGRP8 encode proteins with a bipartite structure consisting of an amino-terminal putative RNA-binding domain and a carboxyl-terminal domain composed of stretches of glycines and serines with interspersed hydrophilic residues. These cDNAs exhibit structural similarity to a class of stress-induced transcripts found in other plant species. The proteins encoded by AtGRP7 and AtGRP8 may be members of a family of proteins which serve a vital role in RNA transcription or processing during stress.
从拟南芥cDNA文库中分离出了两个编码小(-17kDa)富含甘氨酸蛋白(GRPs)的相关DNA序列。AtGRP7和AtGRP8编码的蛋白质具有二分结构,由一个氨基末端假定的RNA结合结构域和一个羧基末端结构域组成,羧基末端结构域由一连串的甘氨酸和丝氨酸以及散布的亲水残基组成。这些cDNA与在其他植物物种中发现的一类应激诱导转录本具有结构相似性。AtGRP7和AtGRP8编码的蛋白质可能是在应激期间对RNA转录或加工起关键作用的蛋白质家族的成员。