Guzmán P, Ecker J R
Department of Biology, University of Pennsylvania, Philadelphia 19104-6018.
Plant Cell. 1990 Jun;2(6):513-23. doi: 10.1105/tpc.2.6.513.
Alterations in the response of dark-grown seedlings to ethylene (the "triple response") were used to isolate a collection of ethylene-related mutants in Arabidopsis thaliana. Mutants displaying a constitutive response (eto1) were found to produce at least 40 times more ethylene than the wild type. The morphological defects in etiolated eto1-1 seedlings reverted to wild type under conditions in which ethylene biosynthesis or ethylene action were inhibited. Mutants that failed to display the apical hook in the absence of ethylene (his1) exhibited reduced ethylene production. In the presence of exogenous ethylene, hypocotyl and root of etiolated his1-1 seedlings were inhibited in elongation but no apical hook was observed. Mutants that were insensitive to ethylene (ein1 and ein2) produced increased amounts of ethylene, displayed hormone insensitivity in both hypocotyl and root responses, and showed an apical hook. Each of the "triple response" mutants has an effect on the shape of the seedling and on the production of the hormone. These mutants should prove to be useful tools for dissecting the mode of ethylene action in plants.
利用黑暗中生长的幼苗对乙烯的反应变化（“三重反应”），在拟南芥中分离出一系列乙烯相关突变体。发现表现出组成型反应的突变体（eto1）产生的乙烯比野生型至少多40倍。在乙烯生物合成或乙烯作用受到抑制的条件下，黄化的eto1-1幼苗的形态缺陷恢复为野生型。在没有乙烯的情况下未能表现出顶端弯钩的突变体（his1）乙烯产量降低。在外源乙烯存在的情况下，黄化的his1-1幼苗的下胚轴和根的伸长受到抑制，但未观察到顶端弯钩。对乙烯不敏感的突变体（ein1和ein2）产生的乙烯量增加，在下胚轴和根的反应中均表现出激素不敏感性，并显示出顶端弯钩。每个“三重反应”突变体都对幼苗的形状和激素的产生有影响。这些突变体应被证明是剖析植物中乙烯作用模式的有用工具。
