University of Miami School of Medicine, Miami, Florida 33136.
Plant Physiol. 1967 Jan;42(1):144-52. doi: 10.1104/pp.42.1.144.
The molecular requirements for ethylene action were investigated using the pea straight growth test. Biological activity requires an unsaturated bond adjacent to a terminal carbon atom, is inversely related to molecular size, and is decreased by substitutions which lower the electron density in the unsaturated position. Evidence is presented that ethylene binds to a metal containing receptor site. CO(2) is a competitive inhibitor of ethylene action, and prevents high concentrations of auxin (which stimulate ethylene formation) from retarding the elongation of etiolated pea stem sections. It is suggested that CO(2) delays fruit ripening by displacing the ripening hormone, ethylene, from its receptor site. Binding of ethylene to the receptor site is also impeded when the O(2) concentration is lowered, and this may explain why fruit ripening is delayed at low O(2) tensions.
使用豌豆直生长试验研究了乙烯作用的分子要求。生物活性需要不饱和键紧邻末端碳原子,与分子大小成反比,并且通过降低不饱和位置的电子密度来降低。有证据表明乙烯与含金属的受体位点结合。CO(2)是乙烯作用的竞争性抑制剂,可防止高浓度的生长素(刺激乙烯形成)阻止黄化豌豆茎段的伸长。有人认为,CO(2)通过从受体部位置换成熟激素乙烯来延迟果实成熟。当 O(2)浓度降低时,乙烯与受体部位的结合也受到阻碍,这可能解释了为什么在低 O(2)张力下果实成熟会延迟。