Department of Pathology, Faculty of Medicine, Garyounis University, Benghazi, Libya.
Libyan J Med. 2011 Mar 31;6. doi: 10.3402/ljm.v6i0.6016.
Among the patients with bladder cancer, a group is still at risk of disease recurrence, progression, and death from their cancer after curative treatment. Angiogenesis is a crucial pathogenic mechanism for this type of urothelial carcinoma and is a potential therapeutic target.
To quantify tumor angiogenesis in bladder cancer and determine whether it correlates with tumor stage and grade.
A series of 42 archival samples from carcinomas of the urinary bladder were graded, staged, and analyzed for microvessel density (MVD) by a double immunohistochemical technique using Factor VIII (FVIII) and CD31 antibodies. The correlation between MVD and histopathological grade and tumor stage was evaluated.
FVIII and CD31 immunoreactivity was observed in 100% of cases and more intensely with CD31. Significantly higher MVD was determined in invasive tumors than in superficial tumors (p<0.05). MVD increased with tumor grade and stage (p<0.05); MVD was not affected by age or sex of the patients.
These data demonstrate that MVD in bladder carcinoma correlates with the tumor grade and stage. Quantification of tumor angiogenesis may allow selection of the type of treatment for bladder cancer patients.
在膀胱癌患者中，有一部分患者在经过根治性治疗后仍存在癌症复发、进展和死亡的风险。血管生成是这种尿路上皮癌的一个关键发病机制，也是潜在的治疗靶点。
定量检测膀胱癌中的肿瘤血管生成，并确定其与肿瘤分期和分级的相关性。
对一系列 42 例膀胱癌存档样本进行分级、分期，并使用 FVIII 和 CD31 抗体的双重免疫组织化学技术分析微血管密度（MVD）。评估 MVD 与组织病理学分级和肿瘤分期之间的相关性。
在 100%的病例中观察到 FVIII 和 CD31 免疫反应，CD31 的反应更强烈。侵袭性肿瘤的 MVD 明显高于表浅性肿瘤（p<0.05）。MVD 随肿瘤分级和分期的增加而增加（p<0.05）；MVD 不受患者年龄和性别影响。
这些数据表明，膀胱癌中的 MVD 与肿瘤分级和分期相关。肿瘤血管生成的定量检测可能有助于选择膀胱癌患者的治疗类型。
