Department of Emergency, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Chin J Integr Med. 2012 May;18(5):391-4. doi: 10.1007/s11655-011-0700-8. Epub 2011 Apr 26.
To observe the effect of andrographolide on the activation of mitogen-activated protein kinases (MAPKs) and expression of nuclear factor-κB (NF-κB) in macrophage foam cells.
The mouse peritoneal macrophages were cultured in the media in the presence of oxidized low-density lipoprotein (ox-LDL), ox-LDL+andrographolide, or neither (control). The phosphorylation of MAPK molecules (p38MAPK, JNK, ERK1/2) and the expressions of NK-κB p65 were examined by Western blot.
As compared with cells in the control group, the expressions of phospho-p38 and NF-κB p65 were increased in the cells cultured with either ox-LDL or ox-LDL+andrographolide (P<0.01), but attenuated significantly in the presence of ox-LDL+ andrographolide when compared with ox-LDL (P<0.05). The phospho-JNK increased in the presence of either ox-LDL or ox-LDL+andrographolide when compared with control cells (P<0.01), but no significant difference existed between ox-LDL and ox-LDL+andrographolide (P>0.05). The expression of phospho-ERK1/2 was increased in the presence of ox-LDL compared with the control cells (P<0.01), but no significant differences existed between the cells cultured in the presence of ox-LDL+andrographolide and the control medium (P>0.05).
Andrographolide could inhibit the activation of ERK1/2, p38MAPK and NK-κB induced by ox-LDL in macrophage foam cells, which might be one of its mechanisms in preventing atherosclerosis.
观察穿心莲内酯对巨噬细胞泡沫细胞中丝裂原活化蛋白激酶（MAPK）的激活和核因子-κB（NF-κB）表达的影响。
用氧化型低密度脂蛋白（ox-LDL）、ox-LDL+穿心莲内酯或无处理（对照）培养小鼠腹腔巨噬细胞。用 Western blot 检测 MAPK 分子（p38MAPK、JNK、ERK1/2）的磷酸化和 NF-κB p65 的表达。
与对照组细胞相比，ox-LDL 或 ox-LDL+穿心莲内酯培养的细胞中磷酸化 p38 和 NF-κB p65 的表达增加（P<0.01），但与 ox-LDL 相比，ox-LDL+穿心莲内酯存在时明显减弱（P<0.05）。与对照组细胞相比，ox-LDL 或 ox-LDL+穿心莲内酯存在时磷酸化 JNK 增加（P<0.01），但 ox-LDL 与 ox-LDL+穿心莲内酯之间无显著差异（P>0.05）。与对照组细胞相比，ox-LDL 存在时磷酸化 ERK1/2 的表达增加（P<0.01），但 ox-LDL+穿心莲内酯培养的细胞与对照培养基之间无显著差异（P>0.05）。
穿心莲内酯可抑制 ox-LDL 诱导的巨噬细胞泡沫细胞中 ERK1/2、p38MAPK 和 NF-κB 的激活，这可能是其预防动脉粥样硬化的机制之一。
