Ding Yongbo, Shi Cunxian, Chen Linjing, Ma Piliang, Li Kezhong, Jin Jin, Zhang Qingfeng, Li Aizhi
Department of Anesthesiology, School of Medicine, Shandong University, Jinan, Shandong 250012, P.R. China.
Department of Anesthesiology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China.
Oncol Lett. 2017 Dec;14(6):7367-7373. doi: 10.3892/ol.2017.7088. Epub 2017 Sep 27.
The present study investigated the effects of andrographolide on postoperative cognitive dysfunction (POCD) in aged rats to gain insight of the underlying mechanism, which may provide theoretical basis for the clinical application of andrographolide to prevent POCD in older patients. Thirty aged male rats were randomly assigned to 3 groups: Control, model and andrographolide groups. The Morris water maze test was used to examine the spatial memory and learning ability of the rats postoperatively. The histological alterations of neuronal cells in the hippocampus were visualized by H&E staining. The serum levels of neuron-specific enolase (NSE), human soluble protein-100β (S-100β) and the inflammation factors of interluekin (IL)-1β, IL-6 and TNF-α involved in the nuclear factor κB (NF-κB)/mitogen-activated protein kinase (MAPK) signaling pathway were detected by ELISA. The NF-κB/MAPK signaling pathway-associated proteins in rat serum were detected by western blotting. Following andrographolide treatment, the rats significantly gained learning ability after surgery. Is it ameliorated hippocampal neuronal injury in rats following surgery. Andrographolide decreased NSE, S-100β, and the inflammation factors, IL-6, IL-1β and TNF-α in serum. Andrographolide reduced NF-κB/MAPK pathway-associated protein expression. Andrographolide ameliorated POCD in aged rats following surgery. The underlying mechanism may be associated with the downregulation the inflammatory factors and NF-κB/MAPK-associated protein expression.
本研究探讨了穿心莲内酯对老年大鼠术后认知功能障碍（POCD）的影响，以深入了解其潜在机制，这可能为穿心莲内酯在老年患者中预防POCD的临床应用提供理论依据。30只老年雄性大鼠随机分为3组：对照组、模型组和穿心莲内酯组。采用Morris水迷宫试验检测大鼠术后的空间记忆和学习能力。通过苏木精-伊红（H&E）染色观察海马神经元细胞的组织学改变。采用酶联免疫吸附测定（ELISA）法检测血清中神经元特异性烯醇化酶（NSE）、人可溶性蛋白100β（S-100β）以及参与核因子κB（NF-κB）/丝裂原活化蛋白激酶（MAPK）信号通路的炎症因子白细胞介素（IL）-1β、IL-6和肿瘤坏死因子-α（TNF-α）的水平。采用蛋白质免疫印迹法检测大鼠血清中与NF-κB/MAPK信号通路相关的蛋白。穿心莲内酯治疗后，大鼠术后学习能力明显提高。它改善了大鼠术后海马神经元损伤。穿心莲内酯降低了血清中NSE、S-100β以及炎症因子IL-6、IL-1β和TNF-α的水平。穿心莲内酯降低了NF-κB/MAPK通路相关蛋白的表达。穿心莲内酯改善了老年大鼠术后的POCD。其潜在机制可能与炎症因子及NF-κB/MAPK相关蛋白表达的下调有关。
