暗场照明反射式光纤束内窥镜显微镜。
Dark-field illuminated reflectance fiber bundle endoscopic microscope.
机构信息
Department of Electrical and Computer Engineering, Johns Hopkins University, 3400 North Charles Street, Barton Hall, Baltimore, Maryland 21218, USA.
出版信息
J Biomed Opt. 2011 Apr;16(4):046003. doi: 10.1117/1.3560298.
Abstract
We propose a reflectance fiber bundle microscope using a dark-field illumination configuration for applications in endoscopic medical imaging and diagnostics. Our experiment results show that dark-field illumination can effectively suppress strong specular reflection from the proximal end of the fiber bundle. We realized a lateral resolution of 4.4 μm using the dark-field illuminated fiber bundle configuration. To demonstrate the feasibility of using the system to study cell morphology, we obtained still and video images of two thyroid cancer cell lines. Our results clearly allow differentiation of different cancer cell types.
摘要
我们提出了一种使用暗场照明配置的反射光纤束显微镜，用于内窥镜医学成像和诊断应用。我们的实验结果表明，暗场照明可以有效地抑制光纤束近端的强镜面反射。我们使用暗场照明光纤束配置实现了 4.4μm 的横向分辨率。为了证明该系统用于研究细胞形态的可行性，我们获得了两种甲状腺癌细胞系的静态和视频图像。我们的结果清楚地允许区分不同的癌细胞类型。
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