通过束状光纤耦合显微镜对生物发光报告基因小鼠的多个器官进行体内生物发光和反射成像。
In vivo bioluminescence and reflectance imaging of multiple organs in bioluminescence reporter mice by bundled-fiber-coupled microscopy.
作者信息
Ando Yoriko, Sakurai Takashi, Koida Kowa, Tei Hajime, Hida Akiko, Nakao Kazuki, Natsume Mistuo, Numano Rika
机构信息
Electronics-Inspired Interdisciplinary Research Institute (EIIRIS), Toyohashi University of Technology, Toyohashi, Aichi, 441-8580, Japan.
Electronics-Inspired Interdisciplinary Research Institute (EIIRIS), Toyohashi University of Technology, Toyohashi, Aichi, 441-8580, Japan; Juntendo University, Bunkyo-ku, Tokyo, 113-8421, Japan.
出版信息
Biomed Opt Express. 2016 Feb 23;7(3):963-78. doi: 10.1364/BOE.7.000963. eCollection 2016 Mar 1.
Bioluminescence imaging (BLI) is used in biomedical research to monitor biological processes within living organisms. Recently, fiber bundles with high transmittance and density have been developed to detect low light with high resolution. Therefore, we have developed a bundled-fiber-coupled microscope with a highly sensitive cooled-CCD camera that enables the BLI of organs within the mouse body. This is the first report of in vivo BLI of the brain and multiple organs in luciferase-reporter mice using bundled-fiber optics. With reflectance imaging, the structures of blood vessels and organs can be seen clearly with light illumination, and it allowed identification of the structural details of bioluminescence images. This technique can also be applied to clinical diagnostics in a low invasive manner.
生物发光成像（BLI）用于生物医学研究，以监测活体内的生物过程。最近，已开发出具有高透光率和密度的光纤束，用于高分辨率检测弱光。因此，我们开发了一种带有高灵敏度冷却电荷耦合器件（CCD）相机的光纤耦合显微镜，可对小鼠体内的器官进行生物发光成像。这是首次使用光纤束对荧光素酶报告基因小鼠的大脑和多个器官进行体内生物发光成像的报告。通过反射成像，在光照下可以清晰地看到血管和器官的结构，并且可以识别生物发光图像的结构细节。该技术还可以以低侵入性方式应用于临床诊断。
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