Choi Hyeong-Jwa, Choi Woo-Sung, Park Jin-Yeon, Kang Kyeong-Hyeon, Prabagar Miglena G, Shin Chan Young, Kang Young-Sun
Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology, Konkuk University, Seoul, Republic of Korea.
Hybridoma (Larchmt). 2011 Apr;30(2):109-16. doi: 10.1089/hyb.2010.0093.
Dextran was used to explore a novel method of enhancing an immune response against T-cell independent type 2 (TI-2) polysaccharide antigens, because of its suitability as a model for the immunogenecity of many TI-2 polysaccharide antigens and its high affinity to SIGN-R1. Here we showed that the primary immune response of IgM, IgG3, and IgG2b was enhanced by dextran in SIGN-R1 knock-out (KO) mice, further evoking the induction of a secondary immune response to IgG2b in parallel. On the other hand, an immune response of IgG1 and IgG2b against T-cell dependent (TD) antigen was strongly enhanced by the administration of ovalbumin (OVA) in SIGN-R1 KO mice. These results indicate that SIGN-R1 is critical in the regulation of immune responses. Therefore, our study suggests that inhibition of TI-2 polysaccharide antigen uptake in SIGN-R1(+) macrophages contributes to the development of novel vaccination strategies against TI-2 polysaccharide antigens.
由于葡聚糖适合作为许多T细胞非依赖性2型（TI-2）多糖抗原免疫原性的模型，且对SIGN-R1具有高亲和力，因此被用于探索增强针对TI-2多糖抗原的免疫反应的新方法。在此我们表明，葡聚糖可增强SIGN-R1基因敲除（KO）小鼠中IgM、IgG3和IgG2b的初次免疫反应，并同时进一步引发对IgG2b的二次免疫反应。另一方面，在SIGN-R1 KO小鼠中，通过给予卵清蛋白（OVA）可强烈增强IgG1和IgG2b对T细胞依赖性（TD）抗原的免疫反应。这些结果表明SIGN-R1在免疫反应调节中起关键作用。因此，我们的研究表明，抑制SIGN-R1(+)巨噬细胞对TI-2多糖抗原的摄取有助于开发针对TI-2多糖抗原的新型疫苗策略。
