从印度单峰驼（Camelus dromedarius）中克隆和序列分析 IL-2、IL-4 和 IFN-γ。
Cloning and sequence analysis of IL-2, IL-4 and IFN-γ from Indian Dromedary camels (Camelus dromedarius).
机构信息
National Research Centre on Camel, Post Bag No. 7, Jorbeer, Bikaner 334 001, Rajasthan, India.
出版信息
Res Vet Sci. 2012 Jun;92(3):420-6. doi: 10.1016/j.rvsc.2011.03.028. Epub 2011 May 6.
The cDNAs of three cytokines, viz., IL-2, IL-4 and IFN-γ from Dromedary camels were amplified by PCR using Bactrian camel sequences and subsequently cloned for sequence analysis. Relationship based on amino acid sequences revealed that Dromedary camel IL-2 shared 99.5% and 99.3% identity at the nucleotide and amino acid levels with Bactrian camel IL-2. In the case of IL-4, the identity of Dromedary camel was 99.7% and 99.2% at the nucleotide and amino acid levels, respectively with that of Bactrian camel. The Dromedary camel IFN-γ shared 100% identity both at nucleotide and amino acid levels with Bactrian camel IFN-γ. Phylogenetic analysis based on amino acid sequences indicated the close relationship in these cytokine genes between the Dromedary camel and other camelids.
使用双峰驼序列通过 PCR 扩增了三种细胞因子（IL-2、IL-4 和 IFN-γ）的 cDNA，随后进行克隆以进行序列分析。基于氨基酸序列的关系表明，Dromedary 骆驼的 IL-2 在核苷酸和氨基酸水平上与 Bactrian 骆驼的 IL-2 具有 99.5%和 99.3%的同源性。在 IL-4 的情况下，Dromedary 骆驼的核苷酸和氨基酸水平的同一性分别为 99.7%和 99.2%，与 Bactrian 骆驼的同一性。Dromedary 骆驼的 IFN-γ 在核苷酸和氨基酸水平上均与 Bactrian 骆驼的 IFN-γ 具有 100%的同一性。基于氨基酸序列的系统发育分析表明，这些细胞因子基因在 Dromedary 骆驼和其他骆驼科动物之间具有密切的关系。
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