National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine, China Agricultural University, No.2 Yuanmingyuan West Road, Haidian District, Beijing 100193, China.
Gene. 2012 Jan 10;491(2):256-9. doi: 10.1016/j.gene.2011.10.007. Epub 2011 Oct 13.
Up to now, little is known about the prion protein gene (PRNP) of domestic bactrian camels, and no polymorphisms of the bactrian camel PRNP have been analyzed or reported. In this study, we cloned and analyzed the PRNP sequences of 89 domestic bactrian camels. The results showed that the amino acid sequence of bactrian camel PrP starts with the consensus sequence MVKSH, with almost identical amino acid sequence to the PrP of dromedary camels. A four octapeptide PHGGGWGQ repeat region follows a nonapeptide (PQGGGGWGQ) in the N-terminal of deduced amino acid sequence from residues 54 to 95. Polymorphisms of PRNP in both species of camels were observed in codons 16(A→V), 17(M→T), 120(N→S), 176(R→K), 215(I→V), 234(S→Y), 237(Y→S), and 239(Q→G) by comparing with other ruminants. The PrP gene nucleotide sequence alignments of bactrian camels (HQ204566.1 and HQ204567.1) showed high identity with dromedary camel (99.2%, 99.1%), sheep (91.9%, 91.8%) and cattle (91.8%, 91.6%). This study provides valuable data for future research on susceptibility or resistance of camels to prion diseases.
迄今为止，有关家养双峰驼朊病毒蛋白基因（PRNP）的信息知之甚少，也尚未分析或报道过双峰驼 PRNP 的多态性。本研究克隆并分析了 89 头家养双峰驼的 PRNP 序列。结果表明，双峰驼 PrP 的氨基酸序列以 MVKSH 共识序列开头，与单峰驼的 PrP 氨基酸序列几乎完全相同。从第 54 位到第 95 位氨基酸的推导序列中，N 端紧随一个四肽重复区（PHGGGWGQ）和一个九肽（PQGGGGWGQ）。通过与其他反刍动物比较，在骆驼的 PRNP 基因中观察到了密码子 16（A→V）、17（M→T）、120（N→S）、176（R→K）、215（I→V）、234（S→Y）、237（Y→S）和 239（Q→G）的多态性。与单峰驼（99.2%，99.1%）、绵羊（91.9%，91.8%）和牛（91.8%，91.6%）相比，双峰驼的 PrP 基因核苷酸序列（HQ204566.1 和 HQ204567.1）具有高度的同源性。本研究为进一步研究骆驼对朊病毒病的易感性或抗性提供了有价值的数据。
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