Ghazal P, Lubon H, Reynolds-Kohler C, Hennighausen L, Nelson J A
Department of Immunology, Scripps Clinic and Research Foundation, La Jolla, California 92037.
Virology. 1990 Jan;174(1):18-25. doi: 10.1016/0042-6822(90)90049-w.
Transcription from the human cytomegalovirus major immediate-early promoter is dependent on host-cell regulatory proteins. The interactions between cellular nuclear proteins and a unique sequence located from nucleotide position -660 to -540 was investigated. The unique region presents a defined target for multiple distinct DNA-binding proteins which appear, in part, to have overlapping binding sites. A minimum of five sequence-specific DNA-binding activities that interact with sequences between -632 and -602, -602 and -557, -602 and -590, -563 and -540, and -602 and -582 were detected. Evidence is presented to suggest that the -632 to -602 site, a previously characterized nuclear factor 1 binding site, does not bind NF1 but strongly interacts with a distinct cellular factor. The binding of cellular proteins to the unique sequence region was shown to be important in directing transcription from the major immediate-early promoter.
人巨细胞病毒主要立即早期启动子的转录依赖于宿主细胞调节蛋白。研究了细胞核蛋白与位于核苷酸位置-660至-540的独特序列之间的相互作用。该独特区域是多个不同DNA结合蛋白的明确靶点，这些蛋白部分似乎具有重叠的结合位点。检测到至少五种与-632至-602、-602至-557、-602至-590、-563至-540以及-602至-582之间序列相互作用的序列特异性DNA结合活性。有证据表明，-632至-602位点（一个先前已表征的核因子1结合位点）不结合NF1，而是与一种不同的细胞因子强烈相互作用。细胞蛋白与独特序列区域的结合在指导主要立即早期启动子的转录中很重要。
