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转录因子与人巨细胞病毒主要立即早期基因启动子调控区域之间的特异性相互作用。

Specific interactions between transcription factors and the promoter-regulatory region of the human cytomegalovirus major immediate-early gene.

作者信息

Ghazal P, Lubon H, Hennighausen L

机构信息

Laboratory of Biochemistry and Metabolism, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892.

出版信息

J Virol. 1988 Mar;62(3):1076-9. doi: 10.1128/JVI.62.3.1076-1079.1988.

DOI:10.1128/JVI.62.3.1076-1079.1988
PMID:2828657
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC253671/
Abstract

Repeat sequence motifs as well as unique sequences between nucleotides -150 and -22 of the human cytomegalovirus immediate-early 1 gene interact in vitro with nuclear proteins. We show that a transcriptional element between nucleotides -91 and -65 stimulated promoter activity in vivo and in vitro by binding specific cellular transcription factors. Finally, a common sequence motif, (T)TGG/AC, present in 15 of the determined binding sites suggests a particular class of nuclear factors associated with the immediate-early 1 gene.

摘要

人巨细胞病毒立即早期1基因核苷酸-150至-22之间的重复序列基序以及独特序列在体外与核蛋白相互作用。我们发现，核苷酸-91至-65之间的转录元件通过结合特定的细胞转录因子在体内和体外刺激启动子活性。最后，在所确定的15个结合位点中存在的共同序列基序（T）TGG/AC表明与立即早期1基因相关的一类特定核因子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62a2/253671/19839e0e7c39/jvirol00082-0424-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62a2/253671/2a1da5069d56/jvirol00082-0422-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62a2/253671/de92fc57eb2e/jvirol00082-0423-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62a2/253671/19839e0e7c39/jvirol00082-0424-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62a2/253671/2a1da5069d56/jvirol00082-0422-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62a2/253671/de92fc57eb2e/jvirol00082-0423-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/62a2/253671/19839e0e7c39/jvirol00082-0424-a.jpg

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本文引用的文献

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Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.在哺乳动物细胞中表达氯霉素乙酰转移酶的重组基因组。
Mol Cell Biol. 1982 Sep;2(9):1044-51. doi: 10.1128/mcb.2.9.1044-1051.1982.
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Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.从分离的哺乳动物细胞核的可溶性提取物中，RNA聚合酶II进行准确的转录起始。
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The transcription factor YY1 binds to negative regulatory elements in the human cytomegalovirus major immediate early enhancer/promoter and mediates repression in non-permissive cells.转录因子YY1与人巨细胞病毒主要立即早期增强子/启动子中的负调控元件结合，并在非允许细胞中介导基因表达的抑制。
Nucleic Acids Res. 1994 Jul 11;22(13):2453-9. doi: 10.1093/nar/22.13.2453.
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Temporal control of gene expression in transgenic mice by a tetracycline-responsive promoter.通过四环素应答启动子对转基因小鼠基因表达进行时间控制。
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Cell-specific activity of the modulator region in the human cytomegalovirus major immediate-early gene.人巨细胞病毒主要立即早期基因中调节区的细胞特异性活性。
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Human cytomegalovirus ie1 transactivates the alpha promoter-enhancer via an 18-base-pair repeat element.人巨细胞病毒即刻早期蛋白1通过一个18碱基对的重复元件反式激活α启动子-增强子。
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Promoter-regulatory region of the major immediate early gene of human cytomegalovirus.人类巨细胞病毒主要立即早期基因的启动子调控区。
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Binding of transcription factors and creation of a large nucleoprotein complex on the human cytomegalovirus enhancer.转录因子的结合以及在人巨细胞病毒增强子上形成大型核蛋白复合物。
Proc Natl Acad Sci U S A. 1987 Jun;84(11):3658-62. doi: 10.1073/pnas.84.11.3658.
6
Multiple tandemly repeated binding sites for cellular nuclear factor 1 that surround the major immediate-early promoters of simian and human cytomegalovirus.
J Virol. 1987 May;61(5):1559-70. doi: 10.1128/JVI.61.5.1559-1570.1987.
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Nuclear proteins from lactating mammary glands bind to the promoter of a milk protein gene.来自泌乳乳腺的核蛋白与乳蛋白基因的启动子结合。
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Nuclear factor 1 interacts with five DNA elements in the promoter region of the human cytomegalovirus major immediate early gene.核因子1与人巨细胞病毒主要立即早期基因启动子区域的五个DNA元件相互作用。
EMBO J. 1986 Jun;5(6):1367-71. doi: 10.1002/j.1460-2075.1986.tb04368.x.
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Sequence-specific interactions of nuclear factors with the insulin gene enhancer.核因子与胰岛素基因增强子的序列特异性相互作用。
Cell. 1986 Apr 11;45(1):35-44. doi: 10.1016/0092-8674(86)90535-0.
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Activation of the major immediate early gene of human cytomegalovirus by cis-acting elements in the promoter-regulatory sequence and by virus-specific trans-acting components.人巨细胞病毒主要立即早期基因在启动子调控序列中的顺式作用元件及病毒特异性反式作用成分作用下的激活
J Virol. 1985 Aug;55(2):431-41. doi: 10.1128/JVI.55.2.431-441.1985.